Cloned (Comment) | Organism |
---|---|
expression of the C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His6-tagged enzyme, mixing of 0.002 ml of 10 mg/mL protein in 100 mM (NH4)2SO4, 20 mM HEPES, pH 7.6, with 0.002 ml reservoir solution containing 15% v/v pentaerythritol ethoxylate, 100 mM HEPES, pH 7.6, 15% v/v MPD, 15°C, 2-3 days, X-ray diffraction structure determination and analysis at 1.76 A resolution, SAD method | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-5-phosphomevalonate | Homo sapiens | - |
ADP + (R)-5-diphosphomevalonate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-5-phosphomevalonate | - |
Homo sapiens | ADP + (R)-5-diphosphomevalonate | - |
? | |
ATP + (R)-5-phosphomevalonate | PMK catalyzes the transfer of the gamma-phosphoryl group of ATP to (R)-5-phosphomevalonate, which results in the formation of ADP and (R)-5-diphosphomevalonate | Homo sapiens | ADP + (R)-5-diphosphomevalonate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | PMK is a member of the NMP superfamily | Homo sapiens |
PMK | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | binding site structure, overview | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | phosphomevalonate kinase catalyzes the rate-limiting step for biosynthesis of isopentenyl diphosphate from mevalonate | Homo sapiens |