Cloned (Comment) | Organism |
---|---|
cloning of cDNAs into pMAL plasmid and expression in Escherichia coli as a fusion protein with MBP tag or hexameric His tag | Sabellastarte spectabilis |
expressed in Escherichia coli | Sabellastarte spectabilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
L-Arg | the reaction mixture contains 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate made up in 100 mM imidazole/HCl (pH 7), 5 mM NADH made up in Tris/HCl (pH 8), pyruvate kinase/lactate dehydrogenase mixture made up in 100 mM imidazole/HCl (pH 7), 100 mM ATP made up in 100 mM imidazole/HCl (pH 7), and recombinant enzyme, at 25°C | Sabellastarte spectabilis | |
13.9 | - |
D-Arg | the reaction mixture contains 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate made up in 100 mM imidazole/HCl (pH 7), 5 mM NADH made up in Tris/HCl (pH 8), pyruvate kinase/lactate dehydrogenase mixture made up in 100 mM imidazole/HCl (pH 7), 100 mM ATP made up in 100 mM imidazole/HCl (pH 7), and recombinant enzyme, at 25°C | Sabellastarte spectabilis | |
34.3 | - |
L-Arg | 25°C | Sabellastarte spectabilis | |
35.4 | - |
L-Arg | 25°C | Sabellastarte spectabilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, Superdex 75 gel filtration | Sabellastarte spectabilis |
42273 | - |
2 * 42273, calculated from sequence | Sabellastarte spectabilis |
42887 | - |
2 * 42887, calculated from sequence | Sabellastarte spectabilis |
42890 | - |
calculated from sequence of cDNA | Sabellastarte spectabilis |
80000 | - |
fusion proteins with hexameric His tag, gel filtration | Sabellastarte spectabilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-Arg | Sabellastarte spectabilis | - |
ADP + omega-N-phospho-D-Arg | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sabellastarte spectabilis | - |
- |
- |
Sabellastarte spectabilis | Q4AED1 | - |
- |
Sabellastarte spectabilis | Q4AED2 | - |
- |
Purification (Comment) | Organism |
---|---|
Amberlite IR120BNA column chromatography and reverse-phase high performance liquid chromatography | Sabellastarte spectabilis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
body wall muscle | - |
Sabellastarte spectabilis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-Arg | 35.2% relative activity compared to L-Arg | Sabellastarte spectabilis | ADP + Nomega-phospho-D-Arg | - |
? | |
ATP + D-Arg | 35.2% of the activity with L-Arg | Sabellastarte spectabilis | ADP + Nomega-phospho-D-Arg | - |
? | |
ATP + D-Arg | - |
Sabellastarte spectabilis | ADP + omega-N-phospho-D-Arg | - |
? | |
ATP + D-Arg | 7.6% of the activity with L-Arg | Sabellastarte spectabilis | ADP + omega-N-phospho-D-Arg | - |
? | |
ATP + L-Arg | - |
Sabellastarte spectabilis | ADP + Nomega-phospho-L-Arg | - |
? | |
ATP + L-Arg | 100% activity | Sabellastarte spectabilis | ADP + Nomega-phospho-L-Arg | - |
? | |
ATP + L-Arg | - |
Sabellastarte spectabilis | ADP + omega-N-phospho-L-Arg | - |
? | |
ATP + lombricine | 0.17% of the activity with L-Arg | Sabellastarte spectabilis | ADP + omega-N-phospholombricine | - |
? | |
ATP + lombricine | 3.0% of the activity with L-Arg | Sabellastarte spectabilis | ADP + omega-N-phospholombricine | - |
? | |
ATP + taurocyamine | 0.11% of the activity with L-Arg | Sabellastarte spectabilis | ADP + N-phosphotaurocyamine | - |
? | |
ATP + taurocyamine | 2.7% of the activity with L-Arg | Sabellastarte spectabilis | ADP + N-phosphotaurocyamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, Superdex 75 gel filtration | Sabellastarte spectabilis |
dimer | 2 * 42273, calculated from sequence | Sabellastarte spectabilis |
dimer | 2 * 42887, calculated from sequence | Sabellastarte spectabilis |
Synonyms | Comment | Organism |
---|---|---|
AK1 | - |
Sabellastarte spectabilis |
AK2 | - |
Sabellastarte spectabilis |
arginine kinase 1 | - |
Sabellastarte spectabilis |
arginine kinase 2 | - |
Sabellastarte spectabilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 3.7 | D-Arg | the reaction mixture contains 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate made up in 100 mM imidazole/HCl (pH 7), 5 mM NADH made up in Tris/HCl (pH 8), pyruvate kinase/lactate dehydrogenase mixture made up in 100 mM imidazole/HCl (pH 7), 100 mM ATP made up in 100 mM imidazole/HCl (pH 7), and recombinant enzyme, at 25°C | Sabellastarte spectabilis | |
5.8 | - |
L-Arg | 25°C | Sabellastarte spectabilis | |
35.4 | - |
L-Arg | the reaction mixture contains 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate made up in 100 mM imidazole/HCl (pH 7), 5 mM NADH made up in Tris/HCl (pH 8), pyruvate kinase/lactate dehydrogenase mixture made up in 100 mM imidazole/HCl (pH 7), 100 mM ATP made up in 100 mM imidazole/HCl (pH 7), and recombinant enzyme, at 25°C | Sabellastarte spectabilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Sabellastarte spectabilis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sabellastarte spectabilis | calculated from sequence | - |
4.2 |
Sabellastarte spectabilis | calculated from sequence of cDNA | - |
6.21 |
Sabellastarte spectabilis | calculated from sequence | - |
7.2 |