Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, optimization of overexpression of full length enzyme and mature enzyme lacking the mitochondrial targeting sequence in Escherichia coli | Chaetopterus variopedatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Chaetopterus variopedatus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
2 * 43000, recombinant enzyme, SDS-PAGE, dimer formation after dissociation of the octamer | Chaetopterus variopedatus |
43000 | - |
8 * 43000, recombinant enzyme, SDS-PAGE, enzyme primarily forms octamers | Chaetopterus variopedatus |
61500 | - |
dimeric enzyme form, gel filtration | Chaetopterus variopedatus |
347000 | - |
octameric enzyme form, gel filtration | Chaetopterus variopedatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + creatine | Chaetopterus variopedatus | the enzyme is involved in energy homeostasis | ADP + phosphocreatine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chaetopterus variopedatus | - |
polychaete worm | - |
Purification (Comment) | Organism |
---|---|
recombinant full length enzyme and mature enzyme from Escherichia colito near homogeneity by affinity and cation exchange chromatography | Chaetopterus variopedatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + creatine | - |
Chaetopterus variopedatus | ADP + phosphocreatine | - |
r | |
ATP + creatine | the enzyme is involved in energy homeostasis | Chaetopterus variopedatus | ADP + phosphocreatine | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43000, recombinant enzyme, SDS-PAGE, dimer formation after dissociation of the octamer | Chaetopterus variopedatus |
More | the octamer-dimer equilibrium highly depends on protein concentration and dilution, respectively, and on temperature, highest percentage of octamer occurs at 28°C, the lowest at 2°C, overview | Chaetopterus variopedatus |
octamer | 8 * 43000, recombinant enzyme, SDS-PAGE, enzyme primarily forms octamers | Chaetopterus variopedatus |
Synonyms | Comment | Organism |
---|---|---|
MtCK | - |
Chaetopterus variopedatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Chaetopterus variopedatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Chaetopterus variopedatus | |
ATP | - |
Chaetopterus variopedatus |