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Literature summary for 2.7.2.8 extracted from

  • Shi, D.; Caldovic, L.; Jin, Z.; Yu, X.; Qu, Q.; Roth, L.; Morizono, H.; Hathout, Y.; Allewell, N.M.; Tuchman, M.
    Expression, crystallization and preliminary crystallographic studies of a novel bifunctional N-acetylglutamate synthase/kinase from Xanthomonas campestris homologous to vertebrate N-acetylglutamate synthase (2006), Acta Crystallogr. Sect. F, F62, 1218-1222.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, selenomethionine substituted enzyme, site directed mutagenesis Xanthomonas campestris

Crystallization (Commentary)

Crystallization (Comment) Organism
of the recombinant wild type protein, the selenomethionine substituted enzyme, the mutants and the methylated enzyme, cocrystallization with ATP, ADP, acetyl-CoA, CoA, N-acetyl-L-glutamate, adenylylimidodiphosphate, arginine Xanthomonas campestris

Protein Variants

Protein Variants Comment Organism
E186A/E387A to improve resolution in crystallization Xanthomonas campestris
E416A/K417A to improve resolution in crystallization Xanthomonas campestris
E94A/K95A to improve resolution in crystallization Xanthomonas campestris
K26A/E27A to improve resolution in crystallization Xanthomonas campestris
K279A/E280A to improve resolution in crystallization Xanthomonas campestris
K419A to improve resolution in crystallization Xanthomonas campestris

Organism

Organism UniProt Comment Textmining
Xanthomonas campestris Q8P8J6
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification methylation of surface lysine residues Xanthomonas campestris

Purification (Commentary)

Purification (Comment) Organism
of the recombinant protein Xanthomonas campestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + L-glutamate
-
 Xanthomonas campestris coenzyme A + N-acetyl-L-glutamate ancestral bifunctional N-acetylglutamate synthase and kinase ?
ATP + N-acetyl-L-glutamate
-
 Xanthomonas campestris ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
acetylglutamate kinase
-
 Xanthomonas campestris
amino-acid acetyltransferase
-
 Xanthomonas campestris
N-acetylglutamate kinase
-
 Xanthomonas campestris
NagK
-
 Xanthomonas campestris
NAGS-K
-
 Xanthomonas campestris