Activating Compound | Comment | Organism | Structure |
---|---|---|---|
beta-Alanine | 10% activation at 0.33 M | Daucus carota |
General Stability | Organism |
---|---|
stable up to. In the DEAE-Sephacel stage of purification, L-lysine and L-proline protect from heat inactivation | Daucus carota |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgSO4 | 15% inhibition | Daucus carota | |
iodosalicylate | 20% inhibition | Daucus carota | |
L-lysine | - |
Daucus carota | |
N-ethylmaleimide | 17% inhibition | Daucus carota | |
p-chloromercuribenzoate | - |
Daucus carota | |
Rose bengal | - |
Daucus carota | |
S-adenosyl-L-methionine | potentiates inhibition by lysine | Daucus carota |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
ATP | pH 7.5, 30°C | Daucus carota | |
2.35 | - |
L-aspartate | pH 7.5, 30°C | Daucus carota |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | does not require monovalent cations for optimum activity | Daucus carota |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
sucrose density gradient centrifugation, peak 1 | Daucus carota |
150000 | - |
sucrose density gradient centrifugation, peak 2 | Daucus carota |
230000 | - |
sucrose density gradient centrifugation, peak 3 | Daucus carota |
246000 | - |
gel filtration, Superose 6 | Daucus carota |
253000 | - |
gel filtration, non-denaturing electrophoresis, 4-20% polyacrylamide gradient gels | Daucus carota |
258000 | - |
gel filtration, Superose 12 | Daucus carota |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daucus carota | - |
carrot | - |
Purification (Comment) | Organism |
---|---|
- |
Daucus carota |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Daucus carota | - |
Storage Stability | Organism |
---|---|
-20°C, can be stored without any noticeable loss in activity | Daucus carota |
use of bicine buffer and addition of sorbitol, bovine serum albumin or poly(ethyleneglycol) 6000 allows storage of the partially purified enzyme as a freeze-dried powder with relatively small loss of activity upon rehydration | Daucus carota |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate | - |
Daucus carota | ADP + 4-phospho-L-aspartate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Daucus carota |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Daucus carota | in presence of 0.1 mM L-lysine, determined by chromatofocusing | - |
4.43 |
Daucus carota | in absence of L-lysine, determined by chromatofocusing | - |
5.16 |