Cloned (Comment) | Organism |
---|---|
transient expression of two mislocalized GFP-tagged PGK mutants NOTP-PGK and NLS-PGK in cytoplasm and nucleus, respectively | Nicotiana benthamiana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of GFP-tagged mutants: the transit peptide deletion mutant NOTP-PGK and the nucleus location mutant NLS-PGK. Generation of PGK-knockdown protoplasts | Nicotiana benthamiana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | chl-PGK is encoded in the nucleus and translated to produce a 50-kD precursor protein and is then processed into mature 43 kD in the chloroplast | Nicotiana benthamiana | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Nicotiana benthamiana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
- |
Nicotiana benthamiana |
50000 | - |
- |
Nicotiana benthamiana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | Nicotiana benthamiana | - |
ADP + 3-phospho-D-glyceroyl phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana benthamiana | Q42961 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | chl-PGK is encoded in the nucleus and translated to produce a 50-kD precursor protein and is then processed into mature 43 kD in the chloroplast | Nicotiana benthamiana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nicotiana benthamiana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | - |
Nicotiana benthamiana | ADP + 3-phospho-D-glyceroyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, premature enzyme, SDS-PAGE, x * 43000, n´mature enzyme, SDS-PAGE | Nicotiana benthamiana |
Synonyms | Comment | Organism |
---|---|---|
chl-PGK | - |
Nicotiana benthamiana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Nicotiana benthamiana |
General Information | Comment | Organism |
---|---|---|
malfunction | reduction of PGK decreases the accumulation of Bamboo mosaic virus BaMV coat protein | Nicotiana benthamiana |
physiological function | phosphoglycerate kinase is an ATP-generating enzyme that acts in the glycolytic, gluconeogenic, and photosynthetic pathways. Chloroplast phosphoglycerate kinase is involved in the targeting of Bamboo mosaic virus to chloroplasts in Nicotiana benthamiana plants, the BaMV replicative intermediate exists in chloroplasts | Nicotiana benthamiana |