Crystallization (Comment) | Organism |
---|---|
NMR solution structure, the GacS periplasmic domain displays a distinct PDC/PAS alpha/beta fold characterized by a three-stranded beta-sheet flanked by alpha-helices and an atypical major loop | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
H124A | no change in overall periplasmic domain fold, mutant shows an altered biofilm morphology compared to the wild-type strain | Pseudomonas aeruginosa |
H133A | no change in overall periplasmic domain fold, mutant abolishes biofilm formation | Pseudomonas aeruginosa |
H97A | mutant abolishes biofilm formation | Pseudomonas aeruginosa |
R94A | mutant strain shows biofilm morphology similar to wild-type | Pseudomonas aeruginosa |
R94A/H97A | no change in overall periplasmic domain fold | Pseudomonas aeruginosa |
W150A | variant is misfolded | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Pseudomonas aeruginosa | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | G3XD98 | - |
- |
Pseudomonas aeruginosa DSM 22644 | G3XD98 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
GacS | - |
Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
physiological function | a GacS mutant devoid of the periplasmic detector domain is severely defective in biofilm formation accompanied by concomitant changes in the expression of small RNAs RsmY/Z that control activation of GacA-regulated genes. Point mutations in a putative ligand binding pocket lined by positively-charged residues originating primarily from the major loop impair biofilm formation | Pseudomonas aeruginosa |