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Literature summary for 2.7.13.3 extracted from

  • Kamikubo, H.; Koyama, T.; Hayashi, M.; Shirai, K.; Yamazaki, Y.; Imamoto, Y.; Kataoka, M.
    The photoreaction of the photoactive yellow protein domain in the light sensor histidine kinase Ppr is influenced by the C-terminal domains (2008), Photochem. Photobiol., 84, 895-902.
    View publication on PubMed
Search for more literature for 2.7.13.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Rhodospirillum centenum
expression of His10-tagged full-length wild-type Ppr in Escherichia coli strain BL21(DE3), overexpression of the PYP domain of Ppr in Escherichia coli, co-expression of the full-length enzyme with tyrosine ammonia-lyase and p-coumaric acid ligase Rhodospirillum centenum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rhodospirillum centenum role of the C-terminal domains in the photocycle of a light sensor histidine kinase Ppr having a photoactive yellow protein, PYP, domain as the photosensor domain, photocycles of the PYP domain of Ppr and of the full-length Ppr. The, overview ?
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 ?

Organism

Organism UniProt Comment Textmining
Rhodospirillum centenum
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Rhodospirillum centenum
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 gene ppr
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Purification (Commentary)

Purification (Comment) Organism
Ni-chelating Sepharose column chromatography and Sephacryl S300 gel filtration Rhodospirillum centenum
recombinant His10-tagged full-length wild-type Ppr from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Rhodospirillum centenum

Renatured (Commentary)

Renatured (Comment) Organism
chemical reconstitution of the recombinantly expressed PYP domain of Ppr with 4-coumaric acid Rhodospirillum centenum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information role of the C-terminal domains in the photocycle of a light sensor histidine kinase Ppr having a photoactive yellow protein, PYP, domain as the photosensor domain, photocycles of the PYP domain of Ppr and of the full-length Ppr. The, overview Rhodospirillum centenum ?
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 ?

Subunits

Subunits Comment Organism
More Ppr is composed of three domains, a PYP domain, a bacteriophytochrome Bph domain, and a histidine kinase domain, in the order from the N- to the C-terminal of Ppr Rhodospirillum centenum

Synonyms

Synonyms Comment Organism
light sensor histidine kinase
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 Rhodospirillum centenum
photoactive yellow protein phytochrome-related protein
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 Rhodospirillum centenum
Ppr
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 Rhodospirillum centenum
PYP phytochrome-related protein
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 Rhodospirillum centenum

pH Range

pH Minimum pH Maximum Comment Organism
5.4 8.1 the light/dark difference spectra of Ppr-PYP are pH sensitive, pH-dependent equilibrium change between Ppr-PYPL and Ppr-PYPM, overview Rhodospirillum centenum