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Literature summary for 2.7.11.7 extracted from
- Specific phosphorylation of threonine by the Dictyostelium myosin II heavy chain kinase family (2001), J. Biol. Chem., 276, 17836-17843.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the catalytic domain of MHCK B as a GST-fusion protein in Escherichia coli BL21(DE3) | Dictyostelium discoideum |
| expression of the His-tagged catalytic domain of MHCK A in Escherichia coli BL21(DE3) | Dictyostelium discoideum |
| expression of the His-tagged catalytic domain of MHCK C in Escherichia coli BL21(DE3) | Dictyostelium discoideum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.28 | - |
RKKFGESEKTKTKEFL-amide | pH 7.0, 25°C | Dictyostelium discoideum |  |
| 0.55 | - |
YAYDTRYRR | pH 7.0, 25°C | Dictyostelium discoideum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Dictyostelium discoideum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin II heavy chain | Dictyostelium discoideum | - |
ADP + myosin II heavy chain phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dictyostelium discoideum | O76739 | gene MHCK C, myosin II heavy chain kinase C | - |
| Dictyostelium discoideum | P42527 | gene MHCK A, myosin II heavy chain kinase A | - |
| Dictyostelium discoideum | P90648 | gene MHCK B, myosin II heavy chain kinase B | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-fusion catalytic domain of MHCK B, expressed in Escherichia coli | Dictyostelium discoideum |
| recombinant His-tagged catalytic domain of MHCK A, expressed in Escherichia coli | Dictyostelium discoideum |
| recombinant His-tagged catalytic domain of MHCK C, expressed in Escherichia coli | Dictyostelium discoideum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + caldesmon | catalytic domain | Dictyostelium discoideum | ADP + caldesmon phosphate | - |
? | |
| ATP + casein | catalytic domain, low activity | Dictyostelium discoideum | ADP + casein phosphate | - |
? | |
| ATP + myelin basic protein | catalytic domain | Dictyostelium discoideum | ADP + myelin basic protein phosphate | - |
? | |
| ATP + myosin II heavy chain | - |
Dictyostelium discoideum | ADP + myosin II heavy chain phosphate | - |
? | |
| ATP + myosin II heavy chain | catalytic domain | Dictyostelium discoideum | ADP + myosin II heavy chain phosphate | - |
? | |
| ATP + myosin II heavy chain | phosphorylates primarly threonine residues | Dictyostelium discoideum | ADP + myosin II heavy chain phosphate | - |
? | |
| ATP + myosin regulatory light chain | catalytic domain | Dictyostelium discoideum | ADP + myosin regulatory light chain phosphate | - |
? | |
| ATP + RKKFGESEKTKTKEFL-amide | low activity | Dictyostelium discoideum | ? | - |
? | |
| ATP + RKKFGESEKTKTKEFL-amide | catalytic domain | Dictyostelium discoideum | ? | - |
? | |
| ATP + RKKFGESEKTKTKEFL-amide | synthetic peptide MH-3 | Dictyostelium discoideum | ? | - |
? | |
| ATP + YAYDTRYRR | consensus sequence | Dictyostelium discoideum | ? | - |
? | |
| ATP + YAYDTRYRR | catalytic domain of the enzyme | Dictyostelium discoideum | ? | - |
? | |
| additional information | localization of autophosphorylation sites | Dictyostelium discoideum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MHCK | - |
Dictyostelium discoideum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Dictyostelium discoideum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Dictyostelium discoideum | |
| additional information | - |
additional information | catalytic domain, several substrates | Dictyostelium discoideum | |
| 0.01 | - |
RKKFGESEKTKTKEFL-amide | pH 7.0, 25°C | Dictyostelium discoideum | |
| 0.55 | - |
RKKFGESEKTKTKEFL-amide | pH 7.0, 25°C | Dictyostelium discoideum | |
| 0.79 | - |
RKKFGESEKTKTKEFL-amide | pH 7.0, 25°C | Dictyostelium discoideum | |
| 14 | - |
YAYDTRYRR | pH 7.0, 25°C | Dictyostelium discoideum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Dictyostelium discoideum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Dictyostelium discoideum | |
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