Literature summary for 2.7.11.32 extracted from

Jiang, L.; Chen, Y.; Zheng, J.; Chen, Z.; Liu, Y.; Tao, Y.; Wu, W.; Chen, Z.; Wang, B.
Structural basis of reversible phosphorylation by maize pyruvate orthophosphate dikinase regulatory protein (2016), Plant Physiol., 170, 732-741 .

Search for more literature for 2.7.11.32

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) RIL cells	Zea mays

Crystallization (Commentary)

Crystallization (Comment)	Organism
using 1.0 M K/Na tartrate 0.1 M MOPS, pH 8.0, 1% (v/v) dioxane, 1 mM AMP, and 2 mM Mg2	Zea mays

Protein Variants

Protein Variants	Comment	Organism
C128S	the activity if the mutant is comparable to that of the wild type enzyme	Zea mays
C165S	the activity if the mutant is comparable to that of the wild type enzyme	Zea mays
D276A/D277A	the mutant exhibits a marked decrease in phosphorylation and dephosphorylation activities as compared to the wild type enzyme	Zea mays
K274A	the mutation exhibits little effect on phosphorylation and dephosphorylation activities as compared to the wild type enzyme	Zea mays
P317F	the mutant exhibits a marked decrease in phosphorylation and dephosphorylation activities as compared to the wild type enzyme	Zea mays

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	10 mM Mg2+ use din assay conditions	Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ADP + [pyruvate phosphate dikinase]	Zea mays	-	AMP + [pyruvate phosphate dikinase] phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Zea mays	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Imac resin column chromatography	Zea mays

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + [pyruvate phosphate dikinase]	-	Zea mays	AMP + [pyruvate phosphate dikinase] phosphate	-	?

Subunits

Subunits	Comment	Organism
homodimer	at pH 7.5. The enzyme mainly exists as a dimer	Zea mays
homotetramer	at pH 8.3	Zea mays

Synonyms

Synonyms	Comment	Organism
PDRP	-	Zea mays
PPDK regulatory protein	-	Zea mays
pyruvate orthophosphate dikinase regulatory protein	-	Zea mays

General Information

General Information	Comment	Organism
metabolism	the enzyme regulates the inorganic phosphate-dependent activation and ADP-dependent inactivation of pyruvate phosphate dikinase by reversible phosphorylation	Zea mays

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Release 2023.1 (January 2023)