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Literature summary for 2.7.11.31 extracted from

  • Ferrer, A.; Hegardt, F.G.
    Phosphorylation of 3-hydroxy-3-methylglutaryl coenzyme A reductase by microsomal 3-hydroxy-3-methylglutaryl coenzyme A reductase kinase (1984), Arch. Biochem. Biophys., 230, 227-237.
    View publication on PubMed
Search for more literature for 2.7.11.31

General Stability

General Stability Organism
about 10% decrease of activity after each freeze-thawing Rattus norvegicus
very stable at either 4°C or -20°C when in microsomes Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
glycerol 25% v/v, reversible inhibition Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-
microsome
-
 Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105000
-
 2 * 105000, SDS-PAGE Rattus norvegicus
205000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] Rattus norvegicus inactivates EC 1.1.1.34 by phosphorylation ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] Rattus norvegicus Sprague-Dawley inactivates EC 1.1.1.34 by phosphorylation ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 Sprague-Dawley
-
Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-80°C, in 0.05 M Tris-HCl buffer, pH 7.4, 0.05 M NaF, 0.003 M EDTA, 0.002 M EGTA, 0.005 M DTT, 0.5 mM PMSF, 10% v/v glycerol, remains stable for at least 3 months Rattus norvegicus
4°C, unstable when solubilized, loses 90% activity within 3 days Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)]
-
 Rattus norvegicus ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] inactivates EC 1.1.1.34 by phosphorylation Rattus norvegicus ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)]
-
 Rattus norvegicus Sprague-Dawley ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] inactivates EC 1.1.1.34 by phosphorylation Rattus norvegicus Sprague-Dawley ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
-
 ?
additional information autophosphorylation in absence of substrate Rattus norvegicus ?
-
 ?
additional information autophosphorylation in absence of substrate Rattus norvegicus Sprague-Dawley ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 105000, SDS-PAGE Rattus norvegicus