Application | Comment | Organism |
---|---|---|
additional information | MEKK1 has a dual function, it can bind to the promoter of WRKY53 and it can interact with the WRKY53 on the protein level and can phosphorylate WRKY53 in vitro thereby increasing its DNA-binding activity. MEKK1 may be able to take a very direct short cut in MAPK signalling by directly phosphorylating a transcription factor | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
full-length cDNA inserted into pGBKT7 vector to generate a MEKK1-GBD fusion construct, introduced into yeast strain Y187 containing the LacZ gene under the control of the GAL1 promoter, full-length MEKK1 fragments subcloned into the pQE30 expression vector to create pQE-MEKK1 encoding the 6 x His-tagged protein and expressed in Escherichia coli, full-length MEKK1 cDNA fragment with a HA-tag cloned into the pGFPc155c vector encoding the C-terminal part of the GFP protein and transformed to Agrobacterium tumefaciens strain GV3101/pMP90 and injected to Arabidopsis leaves | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
K361M | mutation of the kinase domain of MEKK1 reduces the relative expression of the WRKY53 promoter driven GUS gene from 200% in the presence of intact MEKK1 to 132% in the presence of the mutated form | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Arabidopsis thaliana | 5737 | - |
nucleus | MEKK1/WRKY53 complexes are predominantly localized in the nucleus | Arabidopsis thaliana | 5634 | - |
protoplast | - |
Arabidopsis thaliana | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
ecotype Columbia | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epidermal cell | - |
Arabidopsis thaliana | - |
leaf | MEKK1 binds to the promoter of the WRKY53 gene regulating the switch from a leaf age dependent to a plant age dependent expression | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + WRKY53 | phosphorylation increases DNA-binding activity of WRKY53 | Arabidopsis thaliana | ADP + phosphorylated WRKY53 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MEKK1 | - |
Arabidopsis thaliana |
mitogen activated protein kinase kinase kinase | - |
Arabidopsis thaliana |