Activating Compound | Comment | Organism | Structure |
---|---|---|---|
H2O2 | phosphorylation of ASK2 at Thr807 increases in response to H2O2 in cells co-expressing ASK2 and a ASK1 kinase-negative mutant, whereas singly expressed ASK2 does not respond to H2O2. ASK2 kinase-negative mutant co-expressed with ASK1 kinase-negative mutant is not phosphorylated at Thr807 irrespective of the H2O2 treatment | Homo sapiens |
Application | Comment | Organism |
---|---|---|
additional information | ASK2, a highly related serine/threonine kinase to ASK1, functions as a MAP3K only in a heteromeric complex with ASK1 | Homo sapiens |
additional information | ASK2, a highly related serine/threonine kinase to ASK1, functions as a MAP3K only in a heteromeric complex with ASK1 | Mus musculus |
Cloned (Comment) | Organism |
---|---|
full-length ASK2 cDNA inserted in pJG4-5, kinase-negative mutant of ASK1 inserted in pEG202. ASK1 and ASK2 constructs co-transformed along with the reporter plasmid pSH18-34 in EGY48 yeast strains, mutants transfected into HEK293 cells and MEF cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | endogenous ASK2 constitutively degraded in ASK1-deficient cells, kinase-negative mutant of ASK1 effectively activates MAP2K and is more competent to respond to oxidative stress than ASK2 alone. Knockdown of ASK2 reveals that ASK2 is required for oxidative stress-induced JNK activation | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
siRNA | transfection of siRNA for ASK1 into cells reduces expression of not only ASK1 but also ASK2 | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O95382 | - |
- |
Mus musculus | Q9WTR2 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
B-16 cell | - |
Mus musculus | - |
HEK-293 cell | - |
Homo sapiens | - |
L-929 cell | - |
Mus musculus | - |
MEF cell | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ASK1 | ASK2 activates ASK1 by direct phosphorylation | Homo sapiens | ADP + phosphorylated ASK1 | - |
? | |
ATP + ASK1 | ASK2 activates ASK1 by direct phosphorylation | Mus musculus | ADP + phosphorylated ASK1 | - |
? | |
ATP + JNK | activated by ASK2 in complex with ASK1 | Homo sapiens | ADP + phosphorylated JNK | - |
? | |
ATP + MKK4 | activated by ASK2 in complex with ASK1 | Homo sapiens | ADP + phosphorylated MKK4 | - |
? | |
ATP + MKK6 | activated by ASK2 in complex with ASK1 | Homo sapiens | ADP + phosphorylated MKK6 | - |
? | |
ATP + p38 | activated by ASK2 in complex with ASK1 | Homo sapiens | ADP + phosphorylated p38 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
apoptosis signal-regulating kinase 1 | - |
Homo sapiens |
apoptosis signal-regulating kinase 1 | - |
Mus musculus |
apoptosis signal-regulating kinase 2 | - |
Homo sapiens |
apoptosis signal-regulating kinase 2 | - |
Mus musculus |
ASK1 | - |
Homo sapiens |
ASK1 | - |
Mus musculus |
ASK2 | - |
Homo sapiens |
ASK2 | - |
Mus musculus |
MAP3K | - |
Homo sapiens |
MAP3K | - |
Mus musculus |
MAPKKK6 | - |
Homo sapiens |
MAPKKK6 | - |
Mus musculus |
mitogen-activated protein kinase kinase kinase | - |
Homo sapiens |
mitogen-activated protein kinase kinase kinase | - |
Mus musculus |