Literature summary for 2.7.11.25 extracted from

Hu, Q.; Shen, W.; Huang, H.; Liu, J.; Zhang, J.; Huang, X.; Wu, J.; Shi, Y.
Insight into the binding properties of MEKK3 PB1 to MEK5 PB1 from its solution structure (2007), Biochemistry, 46, 13478-13489.

Search for more literature for 2.7.11.25

Application

Application	Comment	Organism
analysis	proline cis-trans isomerization of MEKK3 PB1 of the Gln38-Pro39 bond, first case of structural heterogeneity within PB1 domains, major difference between both isomers in the Pro39 region. Residues Gly37-Leu40 form a type VIb beta-turn in the cis conformation, whereas no obvious character of beta-turn is observed in the trans conformation. Internal motions in the beta3/beta4-turn on a microsecond-millisecond time scale	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
amplified MEKK3 PB1 DNA fragments inserted between NdeI/XhoI sites of pET22b and wild-type MEKK3 PB1 with a C-terminal His tag expressed in Escherichia coli BL21 (DE3) cells, MEKK3 PB1 mutants expressed in Escherichia coli Rosetta cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
K7A	considerably weakens the affinity with MEK5 PB1, dilution is endothermal	Homo sapiens
K7R	sustains the strong binding affinity, Kd is 0.37 micromol, dilution is exothermal like with wild-type	Homo sapiens
R14A	binds to MEK5 PB1 with binding affinity of Kd 0.5 micromol, comparable to that of wild-type, dilution is endothermal	Homo sapiens
R5A	binding affinity decreases greatly to 1.6 micromol, dilution is endothermal	Homo sapiens
R76A	binds to MEK5 PB1 with binding affinity of Kd 0.22 micromol comparable to that of wild-type, dilution is endothermal	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + MEK5	MEKK3 and MEK5 form a heterodimer via PB1-PB1 domain interaction, residues Lys 7 and Arg 5 play important roles in the interaction with MEK5 PB1, MEKK3 PB1 binds MEK5 PB1 tightly with a Kd of about 10-8 M	Homo sapiens	ADP + phosphorylated MEK5	-	?

Subunits

Subunits	Comment	Organism
monomer	in solution, NMR spectroscopy	Homo sapiens

Synonyms

Synonyms	Comment	Organism
MEKK3	-	Homo sapiens
mitogen-activated protein kinase kinase kinase	-	Homo sapiens

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Release 2023.1 (January 2023)