Application | Comment | Organism |
---|---|---|
medicine | Plk1 is a target for the development of anticancer therapeutics | Danio rerio |
Cloned (Comment) | Organism |
---|---|
full-length zPlk1 cDNA and the zPlk1 kinase domain sequence, encompassing residues 17-312, are cloned, the expression plasmid pGEX6P-1 is used | Danio rerio |
Crystallization (Comment) | Organism |
---|---|
the structure of zPlk1 KD in complex with the inhibitor is solved to 2.85 A resolution | Danio rerio |
Protein Variants | Comment | Organism |
---|---|---|
T196D | variant containing an activating substitution | Danio rerio |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-(5-methyl-2-trifluoro-methyl-furan-3-yl)-3-(5-[2-[6-(1H-[1,2,4]triazol-3-ylamino)-pyrimidin-4-ylamino]-ethyl]-thiazol-2-yl)urea | - |
Danio rerio |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34390 | - |
expected molecular weight of the protein with the N-terminal Gly-Pro-Leu-Gly-Ser linker residues and a T196D substitution | Danio rerio |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + protein | Danio rerio | - |
ADP + phosphoprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | Q4KMI8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Danio rerio |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5FAM-RALMEASFADQAR-NH2 | substrate peptide, based on human Cdc25C, used in biochemical activity assay | Danio rerio | ADP + phosphorylated 5FAM-RALMEASFADQAR-NH2 | - |
? | |
ATP + protein | - |
Danio rerio | ADP + phosphoprotein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Plk1 | - |
Danio rerio |
Polo-like kinase 1 | - |
Danio rerio |
zPlk1 | - |
Danio rerio |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
activity assay | Danio rerio |