Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.11.12 extracted from

  • Scholten, A.; Fuss, H.; Heck, A.J.; Dostmann, W.R.
    The hinge region operates as a stability switch in cGMP-dependent protein kinase I alpha (2007), FEBS J., 274, 2274-2286.
    View publication on PubMed
Search for more literature for 2.7.11.12

Activating Compound

Activating Compound Comment Organism Structure
cGMP molecular mechanism and thermodynamics Bos taurus

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of wild-type and mutant enzymes in Spodoptera frugiperda Sf9 cells using the baculovirus infection method Bos taurus

Protein Variants

Protein Variants Comment Organism
additional information construction of a DELTA1-77 truncation mutant Bos taurus
R77L site-directed mutagenesis, the PKGIalpha mutation not only stabilizes the N-terminus but extends a stabilizing effect on the remaining domains of the enzyme as well Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information thermodynamics of PKGIalpha activation by cGMP, overview Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
 2 * 76000, wild-type enzyme, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bos taurus PKGIalpha is a major branch point in the nitric oxide and natriuretic peptide-induced cGMP-signaling pathway. PKG plays a pivotal role in several important biological processes such as the regulation of smooth muscle relaxation, and synaptic plasticity ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 PKGIalpha
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + peptide W15 peptide substrate sequence TQAKRKKSLAMA Bos taurus ADP + phosphorylated peptide W15
-
 ?
additional information PKGIalpha is a major branch point in the nitric oxide and natriuretic peptide-induced cGMP-signaling pathway. PKG plays a pivotal role in several important biological processes such as the regulation of smooth muscle relaxation, and synaptic plasticity Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 76000, wild-type enzyme, SDS-PAGE Bos taurus
More peptide fragment identification by mass spectrometry after limited proteolysis of wild-type PKG in the absence and presence of cGMP, overview Bos taurus

Synonyms

Synonyms Comment Organism
cGMP-dependent protein kinase I alpha
-
 Bos taurus
PKG Ialpha
-
 Bos taurus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Bos taurus