Literature summary for 2.7.1.B23 extracted from

Yethon, J.A.; Whitfield, C.
Purification and characterization of WaaP from Escherichia coli, a lipopolysaccharide kinase essential for outer membrane stability (2001), J. Biol. Chem., 276, 5498-5504 .

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.076	-	alpha-L-glycero-D-manno-heptosyl-(1->3)-alpha-L-glycero-D-manno-heptosyl-(1->5)-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-3-O-[(3R)-3-(tetradecanoyloxy)tetradecanoyl]-2-[[(3R)-3-(dodecanoyloxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose	50 mM Tris, pH 8.5, 17.5 mM MgCl2, 0.5% Triton, 0.5 mM dithiothreitol, 35°C	Escherichia coli
0.13	-	ATP	50 mM Tris, pH 8.5, 17.5 mM MgCl2, 0.5% Triton, 0.5 mM dithiothreitol, 35°C	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30872	-	calculated from amino acid sequence	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + alpha-L-glycero-D-manno-heptosyl-(1->3)-alpha-L-glycero-D-manno-heptosyl-(1->5)-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-3-O-[(3R)-3-(tetradecanoyloxy)tetradecanoyl]-2-[[(3R)-3-(dodecanoyloxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose	Escherichia coli	i.e. alpha-Kdo-(2->4)-[alpha-Hep-(1->3)-alpha-Hep-(1->5)]-alpha-Kdo-(2->6)-lipid A	ADP + alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-3-O-[(3R)-3-(tetradecanoyloxy)tetradecanoyl]-2-[[(3R)-3-(dodecanoyloxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P25741	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0086	-	50 mM Tris, pH 8.5, 17.5 mM MgCl2, 0.5% Triton, 0.5 mM dithiothreitol, 35°C	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + alpha-L-glycero-D-manno-heptosyl-(1->3)-alpha-L-glycero-D-manno-heptosyl-(1->5)-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-3-O-[(3R)-3-(tetradecanoyloxy)tetradecanoyl]-2-[[(3R)-3-(dodecanoyloxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose	i.e. alpha-Kdo-(2->4)-[alpha-Hep-(1->3)-alpha-Hep-(1->5)]-alpha-Kdo-(2->6)-lipid A	Escherichia coli	ADP + alpha-L-glycero-D-manno-heptosyl-(1->3)-((4-O-phospho)-alpha-L-glycero-D-manno-heptosyl-(1->5))-[(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)]-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-3-O-[(3R)-3-(tetradecanoyloxy)tetradecanoyl]-2-[[(3R)-3-(dodecanoyloxy)tetradecanoyl]amino]-4-O-phospho-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose	-	?

Synonyms

Synonyms	Comment	Organism
RfaP	-	Escherichia coli
WaaP	-	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	9	-	Escherichia coli

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Release 2023.1 (January 2023)