Literature summary for 2.7.1.91 extracted from

Louie, D.D.; Kisic, A.; Schroepfer, G.J.
Sphingolipid base metabolism. Partial purification and properties of sphinganine kinase of brain (1976), J. Biol. Chem., 251, 4557-4564.

Search for more literature for 2.7.1.91

General Stability

General Stability	Organism
extensive dialysis or dilution in potassium phosphate buffer, 100 mM, pH 7.4, results especially during the early stages of purification in rapid loss of activity	Bos taurus
repeated freezing and thawing results in 20-30% loss of activity	Bos taurus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Bos taurus
Mg2+	optimum concentration: 10 mM	Bos taurus
Mg2+	ATP:Mg2+ ratio 2:1	Bos taurus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
190000	-	gel filtration	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
partial, multiple forms	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Bos taurus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.00375	-	-	Bos taurus

Storage Stability

Storage Stability	Organism
-20°C, 100 mM phosphate buffer, pH 7.4, 1 mM 2-mercaptoethanol, 1 mM EDTA, 20% glycerol, stable for several months	Bos taurus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)