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Literature summary for 2.7.1.86 extracted from
- Synthetic lethal and biochemical analyses of NAD and NADH kinases in Saccharomyces cerevisiae establish separation of cellular functions (2006), J. Biol. Chem., 281, 22439-22445.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Saccharomyces cerevisiae |
| expression in Escherichia coli as N-terminally His-tagged fusion | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
| mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + NADH | Saccharomyces cerevisiae | Pos5 is responsible for all mitochondrial NAD/NADH kinase. Transitory exposure of Pos5 to the cytoplasm is required for the viability of utr1 mutants | ADP + NADPH | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
| Saccharomyces cerevisiae | Q06892 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
| cobalt chelate affinity column chromatography | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.1 | - |
with GTP as phosphate donor | Saccharomyces cerevisiae |
| 0.5 | - |
with CTP as phosphate donor | Saccharomyces cerevisiae |
| 1.2 | - |
with ATP as phosphate donor | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + NAD+ | - |
Saccharomyces cerevisiae | ADP + NADP+ | - |
? | |
| ATP + NADH | - |
Saccharomyces cerevisiae | ADP + NADPH | - |
? | |
| ATP + NADH | phosphorylates NADH with 2% of the specific activity of its NAD kinase activity | Saccharomyces cerevisiae | ADP + NADPH | - |
? | |
| ATP + NADH | possesses 33% activity with NADH and ATP compared to the activity obtained with NAD and ATP | Saccharomyces cerevisiae | ADP + NADPH | - |
? | |
| ATP + NADH | Pos5 is responsible for all mitochondrial NAD/NADH kinase. Transitory exposure of Pos5 to the cytoplasm is required for the viability of utr1 mutants | Saccharomyces cerevisiae | ADP + NADPH | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADH kinase POS5, mitochondrial | - |
Saccharomyces cerevisiae |
| Pos5 | - |
Saccharomyces cerevisiae |
| Utr1 | - |
Saccharomyces cerevisiae |
| YEF1 | - |
Saccharomyces cerevisiae |
| YEL041W | - |
Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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