Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ethanolamine | Homo sapiens | - |
ADP + O-phosphoethanolamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9HBU6 | gene ek1; gene EKI1 | - |
Homo sapiens | Q9NVF9 | gene eki2, splicing variants eki2alpha and eki2beta; gene eki2, splicing variants eki2alpha and eki2beta | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HCT-116 cell | - |
Homo sapiens | - |
Hep-G2 cell | - |
Homo sapiens | - |
Hep-G2 cell | expression levels of splicing variant ek2alpha is repressed in the HepG2 cells | Homo sapiens | - |
MCF-7 cell | - |
Homo sapiens | - |
additional information | expression profile of the enzyme in cancer cell lines, overview | Homo sapiens | - |
additional information | expression profile of the enzyme in cancer cell lines, overview. Splice variant ek2beta exhibits a markedly low mRNA transcript level in all three of the cancer cell lines | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ethanolamine | - |
Homo sapiens | ADP + O-phosphoethanolamine | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | substantial induction of splicing variant ek2alpha mRNA expression in response to trichostatin A treatment in Hep-G2 cells, the elevated ek2alpha mRNA expression may be due to the accumulation of acetylated histones H3 and H4, located around the promoter regions. Trichostatin A may also induce the recruitment of euchromatic markers and RNA polymerase II to the transcription factor complex that binds to the promoter, thus facilitating gene transcription | up |
General Information | Comment | Organism |
---|---|---|
evolution | human ethanolamine kinase exists as three isoforms: EK1, EK2alpha and EK2beta, encoded by two separate genes; ek1, which produces the ek1 transcript variant 1, and ek2, which undergoes alternative splicing to produce ek2alpha (NCBI reference: NM_018208.2) and ek2beta (GenBank reference: AK001623.1) transcript variants. ek2alpha and ek2beta are respectively translated into EK2alpha and EK2beta | Homo sapiens |
metabolism | choline kinase and ethanolamine kinase are enzymes that initiate the first step in the Kennedy pathway, resulting in the biosynthesis of phosphatidylcholine and phosphatidylethanolamine. Expression profiling of ethanolamine kinase in MCF-7, HCT-116 and Hep-G2 cells, and the transcriptional regulation by epigenetic modification | Homo sapiens |
physiological function | splicing variant ek2beta exhibits a markedly low mRNA transcript level in all three of the cancer cell lines examined, thus suggesting that transcription of the ek2beta gene may be negligible and this isoform may not have a significant role in phosphatidyletanolamine synthesis in the three cancer cell lines | Homo sapiens |