Activating Compound | Comment | Organism | Structure |
---|---|---|---|
PEG 6000 | 5-15%, 3-4fold increase in activity, inhibitory above | Rhodothermus phage RM378 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | complete inhibition at 1 mM | Rhodothermus phage RM378 | |
KCl | inhibitory above 10 mM | Rhodothermus phage RM378 | |
Mn2+ | maximum activity at 0.01 mM, inhibitory above 0.1 mM | Rhodothermus phage RM378 | |
NaCl | inhibitory above 10 mM | Rhodothermus phage RM378 | |
PEG 6000 | 5-15%, 3-4fold increase in activity, inhibitory above | Rhodothermus phage RM378 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
r(A20) | pH 6.0, 65°C | Rhodothermus phage RM378 | |
0.0015 | - |
r(A20) | pH 6.0, 65°C | Rhodothermus phage RM378 | |
0.02 | - |
ATP | pH 6.0, 65°C | Rhodothermus phage RM378 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | maximum activity at 0.1 mM | Rhodothermus phage RM378 | |
Mn2+ | maximum activity at 0.01 mM, inhibitory above 0.1 mM | Rhodothermus phage RM378 | |
additional information | dovalent metal ion required | Rhodothermus phage RM378 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodothermus phage RM378 | - |
infecting Rhodothermus marinus, bifunctional enzyme with 5-kinase domain and 3-phosphohydrolase domain | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.67 | - |
substrate r(A20), pH 6.0, 65°C | Rhodothermus phage RM378 |
3.67 | - |
substrate d(A20), pH 6.0, 65°C | Rhodothermus phage RM378 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | acts both on single- and blunt end double-stranded DNA | Rhodothermus phage RM378 | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-RNA | - |
Rhodothermus phage RM378 | ADP + 5'-phospho-RNA | - |
? | |
ATP + d(A20) | - |
Rhodothermus phage RM378 | ADP + 5'-phospho-d(A20) | - |
? | |
ATP + r(A20) | - |
Rhodothermus phage RM378 | ADP + 5'-phospho-r(A20) | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | - |
Rhodothermus phage RM378 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
stable up to | Rhodothermus phage RM378 |
70 | - |
stable for 1 h | Rhodothermus phage RM378 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
r(A20) | pH 6.0, 65°C | Rhodothermus phage RM378 | |
2.6 | - |
r(A20) | pH 6.0, 65°C | Rhodothermus phage RM378 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Rhodothermus phage RM378 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10 | more than 80% of maximum activity | Rhodothermus phage RM378 |