Protein Variants | Comment | Organism |
---|---|---|
D165A | site-directed mutagenesis, increased activity compared to the wild-type, no 3'-phosphatase activity remaining | Tequatrovirus T4 |
D180A | site-directed mutagenesis, slightly higher activity than the wild-type, reduced 3'-phosphatase activity | Tequatrovirus T4 |
D250A | site-directed mutagenesis, increased activity compared to the wild-type , reduced 3'-phosphatase activity | Tequatrovirus T4 |
D254A | site-directed mutagenesis, increased activity compared to the wild-type, nearly no 3'-phosphatase activity remaining | Tequatrovirus T4 |
D278A | site-directed mutagenesis, increased activity compared to the wild-type, no 3'-phosphatase activity remaining | Tequatrovirus T4 |
K125A | site-directed mutagenesis, slightly higher activity than the wild-type | Tequatrovirus T4 |
R122A | site-directed mutagenesis, slightly higher activity than the wild-type, reduced 3'-phosphatase activity | Tequatrovirus T4 |
R126A | site-directed mutagenesis, highly reduced activity | Tequatrovirus T4 |
R176A | site-directed mutagenesis, increased activity compared to the wild-type, highly reduced 3'-phosphatase activity | Tequatrovirus T4 |
R213A | site-directed mutagenesis, increased activity compared to the wild-type, no 3'-phosphatase activity remaining | Tequatrovirus T4 |
R246A | site-directed mutagenesis, increased activity compared to the wild-type, highly reduced 3'-phosphatase activity | Tequatrovirus T4 |
R279A | site-directed mutagenesis, increased activity compared to the wild-type, highly reduced 3'-phosphatase activity | Tequatrovirus T4 |
R36A | site-directed mutagenesis, similar activity than the wild-type | Tequatrovirus T4 |
R38A | site-directed mutagenesis, highly reduced activity, reduced 3'-phosphatase activity | Tequatrovirus T4 |
S84A | site-directed mutagenesis, slightly lower activity than the wild-type, reduced 3'-phosphatase activity | Tequatrovirus T4 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | - |
Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-RNA | Tequatrovirus T4 | involved in repair of broken RNA termini | ADP + 5'-phospho-RNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
from infected Escherichia coli | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA | 5 amino acids, Lys15, Ser116, Asp35, Arg38, and Arg126, comprise the 5'-kinase active site, structure-activity relationships | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-RNA | - |
Tequatrovirus T4 | ADP + 5'-phospho-RNA | - |
? | |
ATP + 5'-dephospho-RNA | involved in repair of broken RNA termini | Tequatrovirus T4 | ADP + 5'-phospho-RNA | - |
? | |
ATP + synthetic oligonucleotide | - |
Tequatrovirus T4 | ADP + oligonucleotide 5'-phosphate | - |
? | |
additional information | enzyme also has DNA 3'-phosphatase activity | Tequatrovirus T4 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme also possesses a 3'-phosphatase activity | Tequatrovirus T4 |