Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NaF | 0.25 M, enhances activity by about 45% | Daucus carota |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Daucus carota | |
cysteine | - |
Daucus carota | |
EDTA | - |
Daucus carota | |
Fe2+ | - |
Daucus carota | |
Fe3+ | - |
Daucus carota | |
glutathione | - |
Daucus carota | |
Iodine | - |
Daucus carota | |
Mn2+ | - |
Daucus carota | |
NaF | 0.25 M, phosphatase is inhibited by less than 10% | Daucus carota | |
Pb2+ | - |
Daucus carota | |
uridine | inhibitor of phosphatase reaction | Daucus carota |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.5 | - |
Phenylphosphate | phosphotransferase reaction with uridine | Daucus carota | |
3.5 | - |
uridine | phosphotransferase reaction with phenyl phosphate | Daucus carota |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
gel filtration, equilibrium sedimentation, sucrose density gradient centrifugation | Daucus carota |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daucus carota | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Daucus carota |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Daucus carota | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
32 | - |
- |
Daucus carota |
Storage Stability | Organism |
---|---|
-20°C, stable for at least 1 months | Daucus carota |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | - |
Daucus carota | ? | - |
? | |
phenyl phosphate + adenosine | - |
Daucus carota | AMP + phenol | - |
? | |
phenyl phosphate + cytidine | - |
Daucus carota | CMP + phenol | - |
? | |
phenyl phosphate + deoxyadenosine | - |
Daucus carota | dAMP + phenol | - |
? | |
phenyl phosphate + deoxycytidine | - |
Daucus carota | dCMP + phenol | - |
? | |
phenyl phosphate + deoxyguanosine | - |
Daucus carota | dGMP + phenol | - |
? | |
phenyl phosphate + deoxyuridine | - |
Daucus carota | dUMP + phenol | - |
? | |
phenyl phosphate + guanosine | - |
Daucus carota | GMP + phenol | - |
? | |
phenyl phosphate + thymidine | - |
Daucus carota | TMP + phenol | - |
? | |
phenyl phosphate + uridine | - |
Daucus carota | 5'-UMP + phenol | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Daucus carota |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
without protecting agent, about one-third of the initial transfer activity is retained whereas the hydrolase function is almost entirely lost after 22 h | Daucus carota |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Daucus carota | intact enzyme | - |
4.1 |
Daucus carota | subunit A | 4.7 | 4.6 |
Daucus carota | subunit B | - |
5.1 |