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Literature summary for 2.7.1.73 extracted from
- Expression of the guanosine-inosine kinase gene from Exiguobacterium acetylicum in Corynebacterium ammoniagenes and phosphorylation of inosine to produce inosine 5'-monophosphate (2011), World J. Microbiol. Biotechnol., 27, 709-712.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the recombinant strain, which expresses gene gsk and has both inosine kinase activity and ATP-regenerating activity, is used to induce the phosphorylation of inosine to produce inosine 5'-monophosphate, which is widely used as a flavor enhancer | Exiguobacterium acetylicum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene gsk, recombinant expression in Corynebacterium ammoniagenes ATP-regenerating strain ATCC 21477, the Escherichia coli trp promoter proves to be most efficient with regard to inducing the expression of gsk, as compared to tac promoter | Exiguobacterium acetylicum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Exiguobacterium acetylicum |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + guanosine | Exiguobacterium acetylicum | - |
ADP + GMP | - |
? | |
| ATP + inosine | Exiguobacterium acetylicum | - |
ADP + IMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Exiguobacterium acetylicum | - |
gene gsk | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + guanosine | - |
Exiguobacterium acetylicum | ADP + GMP | - |
? | |
| ATP + inosine | - |
Exiguobacterium acetylicum | ADP + IMP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| guanosine-inosine kinase | - |
Exiguobacterium acetylicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Exiguobacterium acetylicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Exiguobacterium acetylicum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Exiguobacterium acetylicum | |
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