Cloned (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, enzyme in complex with ADP and shikimate in the absence of Mg2+ using 0.1 M Tris-HCl buffer pH 8.0, 17% PEG 1500 and 0.5-0.7 M LiCl, or enzyme in complex with ADP and Mg2+ in the absence of shikimate using 0.1 M Tris-HCl buffer pH 8.0, 20% PEG 3350 and 0.1 M MgCl2*6H2O | Mycobacterium tuberculosis |
in complex with ADP-shikimate and with MgADP-, at 1.93 A and 2.8 A resolution, respectively. Presence of Mg2+ influences the conformation of the shikimate hydroxyl groups and the position of the side chains of some of the residues of the activesite. Presence of Cl- seems to influence the affinity of ADP and its position in the active site and the opening length of the LID domain. Shikimate binding causes a closing of the LID domain and also seems to influence the crystallographic packing | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cl- | increases the stability of the structure of the enzyme and influences the affinity of ADP for shikimate kinase | Mycobacterium tuberculosis | |
Mg2+ | influences the position of the hydroxyl groups of the shikimate molecule and some of the residues of the active site of the enzyme | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WPY3 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WPY3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + shikimate | - |
Mycobacterium tuberculosis | ADP + shikimate 3-phosphate | - |
? | |
ATP + shikimate | - |
Mycobacterium tuberculosis H37Rv | ADP + shikimate 3-phosphate | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mycobacterium tuberculosis |