Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation by pyruvate, phosphoenolpyruvate, AMP, cAMP, citrate, fructose 6-phosphate, glucose 6-phosphate, 6-phosphogluconate or 2-keto-3-deoxy-6-phosphogluconate | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 2 mM, 45% inhibition, 5 mM ATP completely relieves inhibition | Pseudomonas putida | |
ATP | at a Mg2+/ATP ratio below 2; free form | Pseudomonas putida | |
additional information | not inhibited by 6-phosphogluconate, 2-oxo-3-deoxy-6-phosphogluconate; not inhibited by cAMP, pyruvate, phosphoenolpyruvate; not inhibited by citrate; not inhibited by CTP and UTP; not inhibited by D-fructose 6-phosphate; not inhibited by D-glucose 6-phosphate; not inhibited by phosphate, AMP | Pseudomonas putida | |
SO42- | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
D-Fructose 1-phosphate | pH 8.0, 25°C | Pseudomonas putida | |
0.19 | - |
D-Fructose 1-phosphate | pH 9.0, 25°C | Pseudomonas putida | |
0.41 | - |
ATP | pH 8.0, 25°C | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 30-50 mM, 2.3fold activation | Pseudomonas putida | |
Mg2+ | required for activity | Pseudomonas putida | |
Mg2+ | sigmoidal response, maximal activity at 5 mM | Pseudomonas putida | |
Mg2+ | actual substrate: MgATP2- | Pseudomonas putida | |
additional information | not activated by phosphate | Pseudomonas putida | |
Na+ | 30-50 mM, 1.5fold activation | Pseudomonas putida | |
NH4+ | 30-50 mM, 1.5fold activation | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 1-phosphate | Pseudomonas putida | sole pathway of D-fructose metabolism in several Pseudomonas species | ADP + D-fructose 1,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-Sephadex, partially purified | Pseudomonas putida |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.02 | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
4°C, 0.8-1.3 mg protein/ml in 50 mM phosphate buffer, pH 7, 0.5 mM dithiothreitol, 5-10% loss of activity | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 1-phosphate | - |
Pseudomonas putida | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 1-phosphate | sole pathway of D-fructose metabolism in several Pseudomonas species | Pseudomonas putida | ADP + D-fructose 1,6-bisphosphate | - |
? | |
GTP + D-fructose 1-phosphate | - |
Pseudomonas putida | GDP + D-fructose 1,6-bisphosphate | - |
? | |
ITP + D-fructose 1-phosphate | - |
Pseudomonas putida | IDP + D-fructose 1,6-bisphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10.2 | very low activity at pH 5.5, approx. half-maximal activity at pH 6.3 and pH 10.2 | Pseudomonas putida |