Literature summary for 2.7.1.52 extracted from

Ohashi, H.; Wahl, C.; Ohashi, T.; Elling, L.; Fujiyama, K.
Effective synthesis of guanosine 5'-diphospho-beta-L-galactose using bacterial L-fucokinase/guanosine 5'-diphosphate-L-fucose pyrophosphorylase (2017), Adv. Synth. Catal., 359, 4227-4234 .

No PubMed abstract available

Search for more literature for 2.7.1.52

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Bacteroides fragilis

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP	-	Bacteroides fragilis
GTP	-	Bacteroides fragilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.03	-	L-galactose	at pH 7.5 and 37°C	Bacteroides fragilis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	Mn2+ is the best suitable divalent cation of the L-fucokinase activity	Bacteroides fragilis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-fucose	Bacteroides fragilis	-	ADP + beta-L-fucose 1-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacteroides fragilis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
His Trap column chromatography	Bacteroides fragilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-fucose	-	Bacteroides fragilis	ADP + beta-L-fucose 1-phosphate	-	?
ATP + L-galactose	the enzyme shows a 45times lower affinity towards L-galactose compared to L-fucose	Bacteroides fragilis	ADP + beta-L-galactose 1-phosphate	-	?
additional information	GDP-L-fucose and diphosphate are formed from L-fucose 1-phosphate and GTP by the GDP-L-Fuc pyrophosphorylase activity of the enzyme	Bacteroides fragilis	?	-	-

Synonyms

Synonyms	Comment	Organism
FKP	the enzyme shows L-fucokinase and guanosine 5'-diphosphate-L-fucose pyrophosphorylase activities	Bacteroides fragilis
L-fucokinase	-	Bacteroides fragilis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.195	-	L-galactose	at pH 7.5 and 37°C	Bacteroides fragilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Bacteroides fragilis

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Release 2023.1 (January 2023)