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show all sequences of 2.7.1.47

Purification, properties, and kinetics of D-ribulokinase from Aerobacter aerogenes

Stayton, M.M.; Fromm, H.J.; J. Biol. Chem. 254, 3765-3771 (1979)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
activation
Klebsiella aerogenes
General Stability
General Stability
Organism
D-ribulose stabilizes
Klebsiella aerogenes
EDTA or 2-mercaptoethanol stabilizes during purification
Klebsiella aerogenes
Inhibitors
Inhibitors
Commentary
Organism
Structure
ADP
product inhibition
Klebsiella aerogenes
additional information
no inhibition by D-ribulose 5-phosphate
Klebsiella aerogenes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic study at 28C
Klebsiella aerogenes
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
requirement
Klebsiella aerogenes
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
59000
-
2 * 59000, SDS-PAGE
Klebsiella aerogenes
116000
-
gel filtration
Klebsiella aerogenes
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + D-ribulose
Klebsiella aerogenes
inducible enzyme of ribitol catabolism
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella aerogenes
-
mutant, constitutive for D-ribulokinase
-
Purification (Commentary)
Commentary
Organism
-
Klebsiella aerogenes
Reaction
Reaction
Commentary
Organism
ATP + D-ribulose = ADP + D-ribulose 5-phosphate
random bi bi reaction mechanism
Klebsiella aerogenes
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
152
-
-
Klebsiella aerogenes
Storage Stability
Storage Stability
Organism
4C, about 10% loss of activity within 6 months
Klebsiella aerogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + D-ribulose
-
641636
Klebsiella aerogenes
ADP + D-ribulose 5-phosphate
-
641636
Klebsiella aerogenes
?
ATP + D-ribulose
inducible enzyme of ribitol catabolism
641636
Klebsiella aerogenes
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 59000, SDS-PAGE
Klebsiella aerogenes
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
activation
Klebsiella aerogenes
General Stability (protein specific)
General Stability
Organism
D-ribulose stabilizes
Klebsiella aerogenes
EDTA or 2-mercaptoethanol stabilizes during purification
Klebsiella aerogenes
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ADP
product inhibition
Klebsiella aerogenes
additional information
no inhibition by D-ribulose 5-phosphate
Klebsiella aerogenes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic study at 28C
Klebsiella aerogenes
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
requirement
Klebsiella aerogenes
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
59000
-
2 * 59000, SDS-PAGE
Klebsiella aerogenes
116000
-
gel filtration
Klebsiella aerogenes
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + D-ribulose
Klebsiella aerogenes
inducible enzyme of ribitol catabolism
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Klebsiella aerogenes
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
152
-
-
Klebsiella aerogenes
Storage Stability (protein specific)
Storage Stability
Organism
4C, about 10% loss of activity within 6 months
Klebsiella aerogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + D-ribulose
-
641636
Klebsiella aerogenes
ADP + D-ribulose 5-phosphate
-
641636
Klebsiella aerogenes
?
ATP + D-ribulose
inducible enzyme of ribitol catabolism
641636
Klebsiella aerogenes
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 59000, SDS-PAGE
Klebsiella aerogenes
Other publictions for EC 2.7.1.47
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
641637
Neuberger
Purification and properties of ...
Klebsiella aerogenes
Biochem. J.
193
513-524
1981
-
-
1
-
-
-
-
3
-
-
2
-
-
3
-
-
1
-
-
-
1
1
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
2
-
-
-
-
1
-
-
1
1
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641636
Stayton
Purification, properties, and ...
Klebsiella aerogenes
J. Biol. Chem.
254
3765-3771
1979
1
-
-
-
-
2
2
1
-
1
2
1
-
2
-
-
1
1
-
-
1
1
2
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
2
-
1
-
1
2
1
-
-
-
1
-
-
1
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
641635
Fromm
D-Ribulokinase from Aerobacter ...
Klebsiella aerogenes
J. Biol. Chem.
234
3097-3101
1959
1
-
-
-
-
-
1
2
-
3
-
1
-
2
-
-
1
-
-
-
1
-
3
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
2
-
3
-
1
-
-
-
1
-
-
1
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-