Literature summary for 2.7.1.45 extracted from

Sanchez-Moreno, I.; Trachtmann, N.; Ilhan, S.; Helaine, V.; Lemaire, M.; Guerard-Helaine, C.; Sprenger, G.A.
2-Ketogluconate kinase from Cupriavidusnecator H16 purification, characterization, and exploration of its substrate specificity (2019), Molecules, 24, 2393 .

Search for more literature for 2.7.1.45

Cloned(Commentary)

Cloned (Comment)	Organism
gene kguK, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS	Cupriavidus necator

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	steady-state kinetics of the recombinant enzyme	Cupriavidus necator

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Cupriavidus necator

Organism

Organism	UniProt	Comment	Textmining
Cupriavidus necator	Q0K080	i.e. Ralstonia eutropha strain H16	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS by nickel affinity chromatography and desalting gel filtration	Cupriavidus necator

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 2-dehydro-3-deoxy-D-gluconate	-	Cupriavidus necator	ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate	-	r
additional information	the substrate specificity analysis reveals that three ketoacids (2-dehydro-3-deoxy-D-gluconate, 2-dehydro-D-gulonate, and 2-dehydro-3-deoxy-D-gulonate) with structures close to the natural substrate (2-dehydro-D-gluconate) are successfully phosphorylated at an efficiency lower than or comparable to 2-dehydrogluconate, as depicted by the measured kinetic constant values. Assay method optimization, overview	Cupriavidus necator	?	-	-

Subunits

Subunits	Comment	Organism
?	x * 35700, recombinant His-tagged enzyme, SDS-PAGE	Cupriavidus necator

Synonyms

Synonyms	Comment	Organism
2-dehydrogluconokinase	-	Cupriavidus necator
2-ketogluconate kinase	-	Cupriavidus necator
KGUK	-	Cupriavidus necator
KGUKCnec	-	Cupriavidus necator
More	see also EC 2.7.1.13	Cupriavidus necator

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Cupriavidus necator

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Cupriavidus necator

General Information

General Information	Comment	Organism
physiological function	2-ketogluconate kinase (KGUK) is involved in the glucose and 2-ketogluconate catabolism of several aerobic bacteria, but relatively few bacterial species are able to utilize 2-ketogluconate as the sole carbon source for growth and energy provision	Cupriavidus necator

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Release 2023.1 (January 2023)