Literature summary for 2.7.1.4 extracted from

Yang, J.; Zhu, L.; Cui, W.; Zhang, C.; Li, D.; Ma, B.; Cheng, L.; Ruan, Y.L.; Ma, F.; Li, M.
Increased activity of MdFRK2, a high-affinity fructokinase, leads to upregulation of sorbitol metabolism and downregulation of sucrose metabolism in apple leaves (2018), Hortic. Res., 5, 71 .

Search for more literature for 2.7.1.4

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli with polyhistidine (His) tag	Malus domestica

Inhibitors

Inhibitors	Comment	Organism	Structure
D-fructose	significantly inhibited by high concentrations of D-fructose	Malus domestica

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1	-	D-fructose	pH 8.0, temperature not specified in the publication, MdFRK2	Malus domestica
0.62	-	D-fructose	pH 8.0, temperature not specified in the publication, MdFRK1	Malus domestica

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + D-fructose	Malus domestica	-	ADP + D-fructose 6-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Malus domestica	-	Royal Gala	-

Purification (Commentary)

Purification (Comment)	Organism
-	Malus domestica

Source Tissue

Source Tissue	Comment	Organism	Textmining
fruit	MdFRK2 expression is extremely high in shoot tips and young fruit	Malus domestica	-
shoot	MdFRK2 expression is extremely high in shoot tips and young fruit	Malus domestica	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-fructose	-	Malus domestica	ADP + D-fructose 6-phosphate	-	?
ATP + D-fructose	high specificity for fructose but no affinity for glucose	Malus domestica	ADP + D-fructose 6-phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 60000, SDS-PAGE	Malus domestica

Synonyms

Synonyms	Comment	Organism
MdFRK1	-	Malus domestica
MdFRK2	-	Malus domestica

General Information

General Information	Comment	Organism
metabolism	fructokinase plays roles in regulating sugar and sorbitol metabolism in Rosaceae plants. Increased activity of MdFRK2, a high affinity fructokinase, leads to upregulation of sorbitol metabolism and downregulation of sucrose metabolism in apple leaves	Malus domestica

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Release 2023.1 (January 2023)