General Stability | Organism |
---|---|
ATP and/or sucrose do not stabilize | Corynebacterium ammoniagenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | not | Corynebacterium ammoniagenes | |
AlCl3 | strong | Corynebacterium ammoniagenes | |
Ba2+ | - |
Corynebacterium ammoniagenes | |
CoA | - |
Corynebacterium ammoniagenes | |
Cu2+ | strong | Corynebacterium ammoniagenes | |
D-pantothenate | substrate inhibition, above 0.5 mM | Corynebacterium ammoniagenes | |
dephospho-CoA | - |
Corynebacterium ammoniagenes | |
EDTA | - |
Corynebacterium ammoniagenes | |
Fe2+ | strong | Corynebacterium ammoniagenes | |
HgCl2 | strong | Corynebacterium ammoniagenes | |
additional information | no inhibition by 2,2'-dipyridyl, Ca2+, Cd2+, 3'-AMP, GTP, GDP, ITP, UTP; not inhibitory: 3',5'-ADP | Corynebacterium ammoniagenes | |
Na2HAsO4 | - |
Corynebacterium ammoniagenes | |
NH4+ | - |
Corynebacterium ammoniagenes | |
pantetheine | - |
Corynebacterium ammoniagenes | |
pantetheine 4'-phosphate | weak | Corynebacterium ammoniagenes | |
pantothenic acid 4'-phosphate | - |
Corynebacterium ammoniagenes | |
pantothenoylcysteine 4'-phosphate | weak | Corynebacterium ammoniagenes | |
pantothenyl alcohol | - |
Corynebacterium ammoniagenes | |
Pb2+ | - |
Corynebacterium ammoniagenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
ATP | pH 6.5, 37°C | Corynebacterium ammoniagenes | |
0.067 | - |
pantothenate | pH 6.5, 37°C | Corynebacterium ammoniagenes | |
1 | - |
ATP | pH 6.5, 37°C | Corynebacterium ammoniagenes | |
1 | - |
ATP | ATP in form of MgATP2- | Corynebacterium ammoniagenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activation, can replace Mg2+ | Corynebacterium ammoniagenes | |
Mg2+ | Km-value: 1 mM | Corynebacterium ammoniagenes | |
Mg2+ | requirement, active substrate: MgATP-complex | Corynebacterium ammoniagenes | |
Mn2+ | activation, can replace Mg2+ | Corynebacterium ammoniagenes | |
additional information | no activation by Ca2+, Cd2+, Ba2+, Pb2+, Fe2+ or Cu2+ | Corynebacterium ammoniagenes | |
Ni2+ | activation, can replace Mg2+ with about 50% efficiency | Corynebacterium ammoniagenes | |
Zn2+ | activation, can replace Mg2+ with about 50% efficiency | Corynebacterium ammoniagenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
gel filtration | Corynebacterium ammoniagenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium ammoniagenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Corynebacterium ammoniagenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.034 | - |
pH 6.5, 37°C | Corynebacterium ammoniagenes |
Storage Stability | Organism |
---|---|
4°C, 2 mg protein/ml in 0.01 M phosphate buffer, pH 7, inactivation within 4 days | Corynebacterium ammoniagenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantothenate | - |
Corynebacterium ammoniagenes | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + D-pantothenate | - |
Corynebacterium ammoniagenes | ADP + D-4'-phosphopantothenate | - |
? | |
GTP + pantothenate | phosphorylation at 28% the rate of ATP | Corynebacterium ammoniagenes | GDP + D-4'-phosphopantothenate | - |
? | |
ITP + pantothenate | phosphorylation at 46% the rate of ATP | Corynebacterium ammoniagenes | IDP + D-4'-phosphopantothenate | - |
? | |
pantetheine + ATP | - |
Corynebacterium ammoniagenes | D-4'-phosphopantetheine + ADP | - |
? | |
panthenoylcysteine + ATP | - |
Corynebacterium ammoniagenes | D-4'-phosphopanthenoylcysteine + ADP | - |
? | |
pantothenyl alcohol + ATP | - |
Corynebacterium ammoniagenes | D-4'-phosphopantothenyl alcohol + ADP | - |
? | |
UTP + pantothenate | phosphorylation at 18% the rate of ATP | Corynebacterium ammoniagenes | UDP + D-4'-phosphopantothenate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 37 | - |
Corynebacterium ammoniagenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
10% to 20% loss of activity within 10 min at pH 7.5 to 8, 10 min stable at pH 7, t1/2: 10 min at pH 6.5 | Corynebacterium ammoniagenes |
37 | - |
10 min, 70% loss of activity | Corynebacterium ammoniagenes |
43 | - |
10 min, inactivation | Corynebacterium ammoniagenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Corynebacterium ammoniagenes |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
t1/2: 10 min at 30°C | Corynebacterium ammoniagenes |
7 | - |
10 min stable at 30°C | Corynebacterium ammoniagenes |
7.5 | - |
90% of activity retained after 10 min at 30°C | Corynebacterium ammoniagenes |
8 | - |
80% of activity retained after 10 min at 30°C | Corynebacterium ammoniagenes |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
CoA | reduced form, pH 6.5, 37°C | Corynebacterium ammoniagenes | |
0.037 | - |
pantetheine 4'-phosphate | pH 6.5, 37°C | Corynebacterium ammoniagenes | |
0.067 | - |
CoA | oxidized form, pH 6.5, 37°C | Corynebacterium ammoniagenes | |
0.125 | - |
pantothenic acid 4'-phosphate | pH 6.5, 37°C | Corynebacterium ammoniagenes |