Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Escherichia coli | |
iodoacetate | - |
Escherichia coli | |
additional information | 60% inhibition by antisera prepared in rabbits against crude extracts of glycerate-grown cells; not: NaF | Escherichia coli | |
p-hydroxymercuribenzoate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | divalent cation requirement satisfied by 0.1 M Mg2+ or Co2+ and partially satisfied by Mn2+, Fe2+ or Ca2+ | Escherichia coli | |
Co2+ | divalent cation requirement satisfied by 0.1 M Mg2+ or Co2+ and partially satisfied by Mn2+, Fe2+ or Ca2+ | Escherichia coli | |
Fe2+ | divalent cation requirement satisfied by 0.1 M Mg2+ or Co2+ and partially satisfied by Mn2+, Fe2+ or Ca2+ | Escherichia coli | |
Mg2+ | divalent cation requirement satisfied by 0.1 M Mg2+ or Co2+ and partially satisfied by Mn2+, Fe2+ or Ca2+ | Escherichia coli | |
Mn2+ | divalent cation requirement satisfied by 0.1 M Mg2+ or Co2+ and partially satisfied by Mn2+, Fe2+ or Ca2+ | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
Crook's strain, ATCC 8739 | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
210 | - |
pH 7.4 | Escherichia coli |
Storage Stability | Organism |
---|---|
-40°C, stable for more than 2 years | Escherichia coli |
4°C, highly purified crystalline enzyme, 45% ethanol, stable for several weeks | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-glycerate | - |
Escherichia coli | ADP + 3-phospho-(R)-glycerate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
stable | Escherichia coli |
10 | - |
stable below | Escherichia coli |