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Literature summary for 2.7.1.30 extracted from

  • Jeng, W.Y.; Panjaitan, N.S.D.; Horng, Y.T.; Chung, W.T.; Chien, C.C.; Soo, P.C.
    The negative effects of KPN00353 on glycerol kinase and microaerobic 1,3-propanediol production in Klebsiella pneumoniae (2017), Front. Microbiol., 8, 2441 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.30

Protein Variants

Protein Variants Comment Organism
additional information recombinant expression of GST-tagged wild-type and mutant KPN00353s in Klebsiella pneumoniae strain MGH 78578. Protein pull-down assay, GST-tagged GlpK binds to His-tagged H65D or His-tagged H65E mutant proteins more strongly than to His-tagged H65R or to His-tagged wild-type KPN00353 and binds weakly to His-tagged H65Q. The binding affinity between GlpK and the His-tagged H110Q mutant protein is similar to that between GlpK and His-tagged wild-type KPN00353. Quantification of intracellular G3P in recombinant Klebsiella pneumoniae overexpressing wild-type or variant KPN00353 proteins. Interaction analysis with wild-type and mutant variants of regulator KPN00353, KPN00353 H65 mutants (H65D, H65E, H65Q, and H65R) overexpression leads to altered intracellular glycerol-3-phosphate concentration in the cells compared to wild-type KPN00353 Klebsiella pneumoniae subsp. pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
EIIAMtl an EIIA homologue in the phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS, UniProt ID A6T5D4), negatively regulates the 1,3-propanediol production in Klebsiella pneumoniae, mutational analysis, overview Klebsiella pneumoniae subsp. pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Klebsiella pneumoniae subsp. pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + glycerol Klebsiella pneumoniae subsp. pneumoniae
-
 ADP + sn-glycerol 3-phosphate
-
 ?
ATP + glycerol Klebsiella pneumoniae subsp. pneumoniae ATCC 700721
-
 ADP + sn-glycerol 3-phosphate
-
 ?
ATP + glycerol Klebsiella pneumoniae subsp. pneumoniae MGH 78578
-
 ADP + sn-glycerol 3-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae subsp. pneumoniae A6TFR2
-
-
Klebsiella pneumoniae subsp. pneumoniae ATCC 700721 A6TFR2
-
-
Klebsiella pneumoniae subsp. pneumoniae MGH 78578 A6TFR2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glycerol
-
 Klebsiella pneumoniae subsp. pneumoniae ADP + sn-glycerol 3-phosphate
-
 ?
ATP + glycerol
-
 Klebsiella pneumoniae subsp. pneumoniae ATCC 700721 ADP + sn-glycerol 3-phosphate
-
 ?
ATP + glycerol
-
 Klebsiella pneumoniae subsp. pneumoniae MGH 78578 ADP + sn-glycerol 3-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
GLPK
-
 Klebsiella pneumoniae subsp. pneumoniae
KpGlpK
-
 Klebsiella pneumoniae subsp. pneumoniae

General Information

General Information Comment Organism
metabolism an EIIA homologue in the phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS or KPN00353, UniProt ID A6T5D4), negatively regulates the 1,3-propanediol (1,3-PD) production in Klebsiella pneumoniae, mutational analysis, overview. Residue His65 of KPN00353 is important for binding to GlpK, weak binding to enzyme GlpK mutant H65Q Klebsiella pneumoniae subsp. pneumoniae