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Literature summary for 2.7.1.24 extracted from

  • Shimosaka, T.; Makarova, K.S.; Koonin, E.V.; Atomi, H.
    Identification of dephospho-coenzyme A (dephospho-CoA) kinase in Thermococcus kodakarensis and elucidation of the entire CoA biosynthesis pathway in Archaea (2019), mBio, 10, e01146-19 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.24

Cloned(Commentary)

Cloned (Comment) Organism
gene TK1697, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli Thermococcus kodakarensis

Protein Variants

Protein Variants Comment Organism
additional information disruption of the TK1697 resulting in CoA auxotrophy. The DELTATK1697 mutant is inoculated in ASW-YT-pyruvate-agmatine medium, but no growth is observed. When 1 mM CoA is added to the medium, the growth defect is partially complemented, with lower growth rate and less cell yield than the wild-type strain KPD1. In the strain transformed with pRPETK1697, the growth defect is almost fully complemented Thermococcus kodakarensis

Inhibitors

Inhibitors Comment Organism Structure
additional information excess concentrations of CoA inhibit growth Thermococcus kodakarensis
UTP at high concentrations Thermococcus kodakarensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Thermococcus kodakarensis
0.14
-
 3'-dephospho-CoA pH 8.0, 80°C, recombinant enzyme Thermococcus kodakarensis
0.26
-
 GTP pH 8.0, 80°C, recombinant enzyme Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20800
-
 recombinant enzyme, gel filtration Thermococcus kodakarensis

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis Q5JIY7
-
-
Thermococcus kodakarensis ATCC BAA-918 Q5JIY7
-
-
Thermococcus kodakarensis JCM 12380 Q5JIY7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli to homogeneity Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3'-dephospho-CoA low activity Thermococcus kodakarensis ADP + CoA
-
 ?
ATP + 3'-dephospho-CoA low activity Thermococcus kodakarensis JCM 12380 ADP + CoA
-
 ?
ATP + 3'-dephospho-CoA low activity Thermococcus kodakarensis ATCC BAA-918 ADP + CoA
-
 ?
GTP + 3'-dephospho-CoA gene TK1697 encodes a GTP-dependent dephospho-CoA kinase Thermococcus kodakarensis GDP + CoA
-
 ?
GTP + 3'-dephospho-CoA gene TK1697 encodes a GTP-dependent dephospho-CoA kinase Thermococcus kodakarensis JCM 12380 GDP + CoA
-
 ?
GTP + 3'-dephospho-CoA gene TK1697 encodes a GTP-dependent dephospho-CoA kinase Thermococcus kodakarensis ATCC BAA-918 GDP + CoA
-
 ?
additional information when NADx02, ADP, AMP, adenosine, or ribose are substituted for dephospho-CoA as phosphate acceptors, no detectable amount of GDP is produced, indicating specificity of the TK1697 protein toward dephospho-CoA. GTP is the preferred phosphate donor Thermococcus kodakarensis ?
-
-
additional information when NADx02, ADP, AMP, adenosine, or ribose are substituted for dephospho-CoA as phosphate acceptors, no detectable amount of GDP is produced, indicating specificity of the TK1697 protein toward dephospho-CoA. GTP is the preferred phosphate donor Thermococcus kodakarensis JCM 12380 ?
-
-
additional information when NADx02, ADP, AMP, adenosine, or ribose are substituted for dephospho-CoA as phosphate acceptors, no detectable amount of GDP is produced, indicating specificity of the TK1697 protein toward dephospho-CoA. GTP is the preferred phosphate donor Thermococcus kodakarensis ATCC BAA-918 ?
-
-

Subunits

Subunits Comment Organism
monomer 1 * 19657, sequence calculation Thermococcus kodakarensis

Synonyms

Synonyms Comment Organism
dephospho-CoA kinase
-
 Thermococcus kodakarensis
dephospho-coenzyme A kinase
-
 Thermococcus kodakarensis
DPCK
-
 Thermococcus kodakarensis
GTP-dependent dephospho-CoA kinase UniProt Thermococcus kodakarensis
GTP-dependent DPCK
-
 Thermococcus kodakarensis
TK1697
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 recombinant enzyme Thermococcus kodakarensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 90 over 50% of maximal activity within this range Thermococcus kodakarensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 80 purified recombinant enzyme, pH 8.0, 2 h, no loss of activity Thermococcus kodakarensis
90
-
 purified recombinant enzyme, pH 8.0, half-life at 90°C is 24 min Thermococcus kodakarensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.57
-
 3'-dephospho-CoA pH 8.0, 80°C, recombinant enzyme Thermococcus kodakarensis
6.68
-
 GTP pH 8.0, 80°C, recombinant enzyme Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 recombinant enzyme Thermococcus kodakarensis

pH Range

pH Minimum pH Maximum Comment Organism
7 9 over 60% of maximal activity within this range Thermococcus kodakarensis

General Information

General Information Comment Organism
evolution enzyme DPCK, encoded by the TK1697 gene of Thermococcus kodakarensis is not homologous to the classical DPCK from bacteria and eukaryotes but is distantly related to bacterial and eukaryotic thiamine diphosphokinases. Orthologues of TK1697 are widely distributed in archaea, suggesting that this form of DPCK is responsible for the last step of CoA biosynthesis in most of the archaea Thermococcus kodakarensis
malfunction disruption of the TK1697 results in CoA auxotrophy Thermococcus kodakarensis
metabolism the pathway for CoA biosynthesis in archaea compared to those of plants and bacteria, overview Thermococcus kodakarensis
physiological function TK1697 gene-encoded DPCK contributes to CoA biosynthesis in Thermococcus kodakarensis. CoA is utilized in a wide range of metabolic pathways, and its biosynthesis is essential for life Thermococcus kodakarensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
25.69
-
 GTP pH 8.0, 80°C, recombinant enzyme Thermococcus kodakarensis
39.79
-
 3'-dephospho-CoA pH 8.0, 80°C, recombinant enzyme Thermococcus kodakarensis