Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Leishmania donovani |
Protein Variants | Comment | Organism |
---|---|---|
D16E | specific activity at the highest attainable adenosine concentration is approximately 2.5-fold lower than that of the wild-type enzyme. Non-saturation kinetics of the D16E mutant are indicative of weaker binding | Leishmania donovani |
D16N | kcat/Km value for adenosine is lowered more than 1000fold. Mutation does not significantly affected the apparent Km for ATP | Leishmania donovani |
D16V | kcat/Km value for adenosine is lowered more than 1000fold. Mutation does not significantly affected the apparent Km for ATP | Leishmania donovani |
R131A | mutant shows abrogated AMP-binding, insensitivity towards AMP inhibition, unaltered Km-value for adenosine | Leishmania donovani |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-deoxyadenosine | - |
Leishmania donovani | |
3'-deoxyadenosine | - |
Leishmania donovani | |
AMP | inhibits both D16V and D16N mutant enzymes to similar extents to that observed with the wild-type enzyme, displays a much reduced ability in inhibiting R131A mutant enzyme | Leishmania donovani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
adenosine | wild-type enzyme | Leishmania donovani | |
0.0059 | - |
adenosine | mutant enzyme R131A | Leishmania donovani | |
0.017 | - |
ATP | mutant enzyme D16N | Leishmania donovani | |
0.02 | - |
ATP | mutant enzyme D16V | Leishmania donovani | |
0.036 | - |
ATP | wild-type enzyme | Leishmania donovani | |
0.036 | - |
3'-deoxyadenosine | wild-type enzyme | Leishmania donovani | |
0.05 | - |
ATP | mutant enzyme R131A | Leishmania donovani | |
0.077 | - |
2'-deoxyadenosine | wild-type enzyme | Leishmania donovani | |
1.293 | - |
adenosine | mutant enzyme D16V | Leishmania donovani | |
1.575 | - |
adenosine | mutant enzyme D16N | Leishmania donovani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxy-adenosine | - |
Leishmania donovani | ADP + 2'-deoxy-AMP | - |
? | |
ATP + adenosine | - |
Leishmania donovani | ADP + AMP | - |
? | |
ATP + cordycepin | - |
Leishmania donovani | ADP + cordycepin 5'-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LdAdK | - |
Leishmania donovani |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.733 | - |
ATP | mutant enzyme R131A | Leishmania donovani | |
0.733 | - |
adenosine | mutant enzyme R131A | Leishmania donovani | |
5.1 | - |
ATP | mutant enzyme D16V | Leishmania donovani | |
5.1 | - |
adenosine | mutant enzyme D16V | Leishmania donovani | |
5.2 | - |
ATP | mutant enzyme D16N | Leishmania donovani | |
5.2 | - |
adenosine | mutant enzyme D16N | Leishmania donovani | |
5.25 | - |
2'-deoxyadenosine | wild-type enzyme | Leishmania donovani | |
6.7 | - |
3'-deoxyadenosine | wild-type enzyme | Leishmania donovani | |
18 | - |
ATP | wild-type enzyme | Leishmania donovani | |
18 | - |
adenosine | wild-type enzyme | Leishmania donovani | |
24.15 | - |
ATP | wild-type enzyme | Leishmania donovani | |
24.15 | - |
adenosine | wild-type enzyme | Leishmania donovani |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
3'-deoxyadenosine | wild-type enzyme | Leishmania donovani | |
0.01575 | - |
2'-deoxyadenosine | wild-type enzyme | Leishmania donovani |