Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme activity is increased in plants after inoculation with diverse RNA viruses or a germinivirus lacking an intact L2 protein | Nicotiana benthamiana |
Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis, the His-tagged enzyme is expressed with or without coexpression of viral AL2 and L2 proteins in an enzyme-deficient Escherichia coli strain, enzyme expression leads to complementation of the mutant, coexpression leads to inactivation of the enzyme and no complementation, expression in yeast cells utilizing the two-hybrid system coexpressing the L2 protein of geminivirus, coexpression leads to inactivation of the enzyme | Arabidopsis thaliana |
expression of viral proteins L2 and AL2 in nucelus and cytosol of transgenic plants via the Agrobacterium infection system highly reduces enzyme activity | Nicotiana benthamiana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Arabidopsis thaliana | |
geminivirus AL2 | inactivation of the enzyme in vitro and in vivo via direct interaction, AL2 is a RNA geminivirus of the Begomovirus genera encoded protein serving as pathogenicity determinant causing enhanced susceptibility of target organisms, overview | Arabidopsis thaliana | |
geminivirus AL2 | inactivation of the enzyme in vitro and in vivo via direct interaction, AL2 is a RNA geminivirus of the Begomovirus genera encoded protein serving as pathogenicity determinant causing enhanced susceptibility of target organisms, overview | Nicotiana benthamiana | |
geminivirus L2 | inactivation of the enzyme in vitro and in vivo via direct interaction, L2 is a RNA geminivirus of the Curtovirus genera encoded protein serving as pathogenicity determinant causing enhanced susceptibility of target organisms, overview | Arabidopsis thaliana | |
geminivirus L2 | inactivation of the enzyme in vitro and in vivo via direct interaction, L2 is a RNA geminivirus of the Curtovirus genera encoded protein serving as pathogenicity determinant causing enhanced susceptibility of target organisms, overview | Nicotiana benthamiana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Nicotiana benthamiana | |
Mg2+ | - |
Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine | Nicotiana benthamiana | the enzyme catalyzes the salvage synthesis of 5'-AMP from ADP and ATP | ADP + AMP | - |
? | |
ATP + adenosine | Arabidopsis thaliana | the enzyme catalyzes the salvage synthesis of 5'-AMP from ADP and ATP | ADP + AMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9LZG0 | 2 isozymes ADK1 and ADK2 | - |
Nicotiana benthamiana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially, recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine | - |
Nicotiana benthamiana | ADP + AMP | - |
? | |
ATP + adenosine | - |
Arabidopsis thaliana | ADP + AMP | - |
? | |
ATP + adenosine | the enzyme catalyzes the salvage synthesis of 5'-AMP from ADP and ATP | Nicotiana benthamiana | ADP + AMP | - |
? | |
ATP + adenosine | the enzyme catalyzes the salvage synthesis of 5'-AMP from ADP and ATP | Arabidopsis thaliana | ADP + AMP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADK | - |
Nicotiana benthamiana |
ADK | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Nicotiana benthamiana |
37 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Nicotiana benthamiana |
7.6 | - |
assay at | Arabidopsis thaliana |