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Literature summary for 2.7.1.2 extracted from

  • Conway, L.P.; Liu, F.F.; Li, Q.; Voglmeir, J.
    The Shewanella woodyi galactokinase pool phosphorylates glucose at the 6-position (2018), Carbohydr. Res., 455, 39-44 .
    View publication on PubMed
Search for more literature for 2.7.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Shewanella woodyi

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Shewanella woodyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9
-
 D-glucose pH 7.5, 37°C Shewanella woodyi
3.9
-
 D-glucose pH 7.5, 37°C Shewanella woodyi
19
-
 D-glucose pH 7.5, 37°C Shewanella woodyi

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates, less efficient than Mg2+ Shewanella woodyi
Mg2+ clear requirement for a divalent metal ion and a strong preference for Mg2+ Shewanella woodyi
Mn2+ activates, less efficient than Mg2+ Shewanella woodyi

Organism

Organism UniProt Comment Textmining
Shewanella woodyi B1KD89
-
-
Shewanella woodyi B1KDE7 cf. EC 2.7.1.2
-
Shewanella woodyi B1KRG2 cf. EC 2.7.1.6
-
Shewanella woodyi ATCC 51908 B1KD89
-
-
Shewanella woodyi ATCC 51908 B1KDE7 cf. EC 2.7.1.2
-
Shewanella woodyi ATCC 51908 B1KRG2 cf. EC 2.7.1.6
-

Purification (Commentary)

Purification (Comment) Organism
-
 Shewanella woodyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucose
-
 Shewanella woodyi ADP + D-glucose 6-phosphate
-
 ?
ATP + D-glucose
-
 Shewanella woodyi ATCC 51908 ADP + D-glucose 6-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Sw0678
-
 Shewanella woodyi
Sw2155
-
 Shewanella woodyi
Sw3773
-
 Shewanella woodyi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Shewanella woodyi
42
-
-
 Shewanella woodyi

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 25°C: about 60% of maximal activity, 45°C: about 40% of maximal activity Shewanella woodyi
25 45 25°C: about 65% of maximal activity, 45°C: about 80% of maximal activity Shewanella woodyi
25 50 25°C: about 45% of maximal activity, 50°C: about 25% of maximal activity Shewanella woodyi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.058
-
 D-glucose pH 7.5, 37°C Shewanella woodyi
0.21
-
 D-glucose pH 7.5, 37°C Shewanella woodyi
0.67
-
 D-glucose pH 7.5, 37°C Shewanella woodyi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Shewanella woodyi

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8.5 pH 4.5: about 45% of maximal activity, pH 8.5: about 45% of maximal activity Shewanella woodyi
6 8.5 pH 6.0: about 55% of maximal activity, pH 8.5: about 40% of maximal activity Shewanella woodyi
6.5 8.5 pH 6.5: about 55% of maximal activity, pH 8.5: about 55% of maximal activity Shewanella woodyi

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.01
-
 D-glucose pH 7.5, 37°C Shewanella woodyi
0.014
-
 D-glucose pH 7.5, 37°C Shewanella woodyi
0.74
-
 D-glucose pH 7.5, 37°C Shewanella woodyi