Crystallization (Comment) | Organism |
---|---|
crystal structures of isoform APH(2'')-IVa, wild-type and mutant F95M, in complex with either adenosine or guanosine | Enterococcus casseliflavus |
Protein Variants | Comment | Organism |
---|---|---|
F95M | no significantly different binding affinities as compared with the wild-type | Enterococcus casseliflavus |
F95Y | mutation shifts the nucleotide selectivity from a 2.5fold preference for ATP to a 1.5fold preference for GTP | Enterococcus casseliflavus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.049 | - |
GTP | mutant F95Y, pH 7.5, 37°C | Enterococcus casseliflavus | |
0.055 | - |
ATP | mutant F95M, pH 7.5, 37°C | Enterococcus casseliflavus | |
0.069 | - |
ATP | wild-type, pH 7.5, 37°C | Enterococcus casseliflavus | |
0.072 | - |
ATP | mutant F95Y, pH 7.5, 37°C | Enterococcus casseliflavus | |
0.156 | - |
GTP | mutant F95M, pH 7.5, 37°C | Enterococcus casseliflavus | |
0.168 | - |
GTP | wild-type, pH 7.5, 37°C | Enterococcus casseliflavus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus casseliflavus | O68183 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + tobramycin | - |
Enterococcus casseliflavus | ADP + tobramycin 2''-phosphate | - |
? | |
GTP + tobramycin | - |
Enterococcus casseliflavus | GDP + tobramycin 2''-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
APH(2'')-Id | - |
Enterococcus casseliflavus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.06 | - |
ATP | mutant F95Y, pH 7.5, 37°C | Enterococcus casseliflavus | |
1.15 | - |
GTP | mutant F95Y, pH 7.5, 37°C | Enterococcus casseliflavus | |
3.06 | - |
ATP | mutant F95M, pH 7.5, 37°C | Enterococcus casseliflavus | |
4.35 | - |
GTP | mutant F95M, pH 7.5, 37°C | Enterococcus casseliflavus | |
4.37 | - |
ATP | wild-type, pH 7.5, 37°C | Enterococcus casseliflavus | |
5.03 | - |
GTP | wild-type, pH 7.5, 37°C | Enterococcus casseliflavus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
ATP | mutant F95Y, pH 7.5, 37°C | Enterococcus casseliflavus | |
23 | - |
GTP | mutant F95Y, pH 7.5, 37°C | Enterococcus casseliflavus | |
28 | - |
GTP | mutant F95M, pH 7.5, 37°C | Enterococcus casseliflavus | |
30 | - |
GTP | wild-type, pH 7.5, 37°C | Enterococcus casseliflavus | |
55 | - |
ATP | mutant F95M, pH 7.5, 37°C | Enterococcus casseliflavus | |
63 | - |
ATP | wild-type, pH 7.5, 37°C | Enterococcus casseliflavus |