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Literature summary for 2.7.1.177 extracted from

  • Fan, C.; Bobik, T.A.
    The PduX enzyme of Salmonella enterica is an L-threonine kinase used for coenzyme B12 synthesis (2008), J. Biol. Chem., 283, 11322-11329.
    View publication on PubMed
Search for more literature for 2.7.1.177

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0547
-
 ATP Michaelis-Menten kinetics, pH 7.4, temperature not specified in the publication Salmonella enterica
0.069
-
 ATP calculated from double-reciprocal plots, pH 7.4, temperature not specified in the publication Salmonella enterica
0.127
-
 L-threonine calculated from double-reciprocal plots, pH 7.4, temperature not specified in the publication Salmonella enterica
0.146
-
 L-threonine Michaelis-Menten kinetics, pH 7.4, temperature not specified in the publication Salmonella enterica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32787
-
 x * 32787, calculated from sequence, x * 35000, SDS-PAGE Salmonella enterica
35000
-
 x * 32787, calculated from sequence, x * 35000, SDS-PAGE Salmonella enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-threonine Salmonella enterica
-
 ADP + O-phospho-L-threonine
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica Q9XDM4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Salmonella enterica

Reaction

Reaction Comment Organism Reaction ID
ATP + L-threonine = ADP + O-phospho-L-threonine steady-state ordered BiBI mechanism Salmonella enterica
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-threonine
-
 Salmonella enterica ADP + O-phospho-L-threonine
-
 ?
ATP + L-threonine
-
 Salmonella enterica ADP + O-phospho-L-threonine product indentified by 31P NMR spectroscopy ?
additional information the activity with CTP, GTP, or UTP is 6, 11, 3% of the activity with ATP, respectively Salmonella enterica ?
-
 ?

Subunits

Subunits Comment Organism
? x * 32787, calculated from sequence, x * 35000, SDS-PAGE Salmonella enterica

Synonyms

Synonyms Comment Organism
PduX
-
 Salmonella enterica

General Information

General Information Comment Organism
metabolism involved in the biosynthesis of adenosylcobalamin Salmonella enterica