Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-seryl-tRNASec | Methanocaldococcus jannaschii | - |
ADP + O-phospho-L-seryl-tRNASec | - |
? | |
ATP + L-seryl-tRNASec | Methanocaldococcus jannaschii DSM 2661 | - |
ADP + O-phospho-L-seryl-tRNASec | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58933 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q58933 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-seryl-tRNASec | - |
Methanocaldococcus jannaschii | ADP + O-phospho-L-seryl-tRNASec | - |
? | |
ATP + L-seryl-tRNASec | - |
Methanocaldococcus jannaschii DSM 2661 | ADP + O-phospho-L-seryl-tRNASec | - |
? | |
ATP + L-seryl-tRNASec | PSTK distinguishes tRNASec from tRNASer. Unlike eukaryotic PSTK, the archaeal enzyme recognizes the acceptor stem rather than the length and secondary structure of the D-stem. The seryl moiety of L-seryl-tRNASec is not required for enzyme recognition, as PSTK efficiently phosphorylates L-threonyl-tRNASec | Methanocaldococcus jannaschii | O-phospho-L-seryl-tRNASec + ADP | - |
? | |
ATP + L-seryl-tRNASec | PSTK distinguishes tRNASec from tRNASer. Unlike eukaryotic PSTK, the archaeal enzyme recognizes the acceptor stem rather than the length and secondary structure of the D-stem. The seryl moiety of L-seryl-tRNASec is not required for enzyme recognition, as PSTK efficiently phosphorylates L-threonyl-tRNASec | Methanocaldococcus jannaschii DSM 2661 | O-phospho-L-seryl-tRNASec + ADP | - |
? | |
L-threonyl-tRNASec + ATP | - |
Methanocaldococcus jannaschii | O-phospho-L-threonyl-tRNASec + ADP | - |
? | |
L-threonyl-tRNASec + ATP | - |
Methanocaldococcus jannaschii DSM 2661 | O-phospho-L-threonyl-tRNASec + ADP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PSTK | - |
Methanocaldococcus jannaschii |