Cloned (Comment) | Organism |
---|---|
single copy Itpk2 gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged GmITPK2 in Escherichia coli strain BL21(DE3) | Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | cytosolic localization of GmITPK2 protein is predicted by CELLO tool with a reliability index of 2.976 | Glycine max | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,3,4-trisphosphate | Glycine max | - |
ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,3,4-trisphosphate | Glycine max | - |
ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | A7X665 | cv. Pusa-16, grown in the fields of Division of Genetics, I.A.R.I, New Delhi | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Glycine max | - |
root | - |
Glycine max | - |
seed | spatio-temporal transcript profiling signified GmItpk2 to be seed-specific, with higher transcript levels in the early stage of seed development, GmItpk2 expression profiling overview | Glycine max | - |
seedling | - |
Glycine max | - |
stem | - |
Glycine max | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,3,4-trisphosphate | - |
Glycine max | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,3,4-trisphosphate | - |
Glycine max | ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE | Glycine max |
More | the predicted three-dimensional model confirms 12 alpha-helices and 14 beta-barrel sheets with ATP-binding site close to beta-sheet present towards the C-terminus of the protein molecule. Secondary structure prediction of GmITPK2 using PDBSum tool | Glycine max |
Synonyms | Comment | Organism |
---|---|---|
GmItpk2 | - |
Glycine max |
inositol 1,3,4 tris phosphate 5/6 kinase-2 | - |
Glycine max |
inositol 1,3,4 trisphosphate kinase-2 | - |
Glycine max |
inositol 1,4,5 trisphosphate 3/6 kinase | - |
Glycine max |
Ipk2 | - |
Glycine max |
ITPK2 | - |
Glycine max |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Glycine max |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Glycine max | sequence calculation | - |
5.9 |
Organism | Comment | Expression |
---|---|---|
Glycine max | GmITPK2 is an early dehydration responsive gene which is also induced by dehydration and exogenous treatment with abscisic acid | up |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic tree showing the evolutionary relatedness of GmITPK2 with ITPKs of monocot and dicot species using Entamoeba histolytica (EhITPK) as an outgroup member | Glycine max |
metabolism | following the formation of myo-inositol-3-phosphate, sequential phosphorylation reactions via action of various inositol phosphate kinases-myo-inositol kinase (Mik), inositol 1,3,4-trisphosphate kinase (Itpk), inositol 1,4,5-trisphosphate 3/6 kinase (Ipk2), and inositol 1,3,4,5,6-pentakisphosphate kinase (Ipk1) lead to phytic acid (PA, myo-inositol 1,2,3,4,5,6-hexakisphosphate) synthesis via the lipid-independent pathway and fine-tune the phosphorous flux towards PA accumulation | Glycine max |
additional information | enzyme homology-based modeling and structure comparisons, overview. Active site mapping and molecular docking with ATP | Glycine max |
physiological function | inositol 1,3,4-trisphosphate kinase-2 (GmItpk2), catalyzing the ATP-dependent phosphorylation of inositol 1,3,4-trisphosphate (IP3) to inositol 1,3,4,5-tetraphosphate or inositol 1,3,4,6-tetraphosphate, is a key enzyme diverting the flux of inositol phosphate pool towards phytate biosynthesis | Glycine max |