BRENDA - Enzyme Database show
show all sequences of 2.7.1.159

Specificity determinants in inositol polyphosphate synthesis: crystal structure of inositol 1,3,4-trisphosphate 5/6-kinase

Miller, G.J.; Wilson, M.P.; Majerus, P.W.; Hurley, J.H.; Mol. Cell 18, 201-212 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of GST-tagged wild-type enzyme and of mutant enzymes in Escherichia coli
Entamoeba histolytica
Crystallization (Commentary)
Crystallization
Organism
purified recombinant enzyme free or in complex with ATP analogue AMP-PCP and substrate inositol-1,3,4-trisphosphate in presence of Mg2+, 30 mg/ml protein in 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, and 10 mM DTT, mixed with a solution containing 20% PEG 3000, 0.1 M Tris-HCl, pH 7.5, and 0.1 M Ca(OAc)2, several months, crystals are seeded into sitting drops of 15 mg/ml protein, 25% PEG 3350, 5% PEG 400, 0.1 M Bis-Tris, pH 5.5, 10 mM DTT, and 10 mM L-cysteine, with or without ligands added as 10 mM AMP-PCP, 10 mM MgCl2, and 5 mM inositol-1,3,4-trisphosphate, direct X-ray diffraction analysis after this step or further seeding adding ADP and other ligands, overview, X-ray diffraction structure determination and analysis at 1.2-2.0 A resolution
Entamoeba histolytica
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of diverse mutants and analysis of the function of the residues in catalysis and substrate binding, overview
Entamoeba histolytica
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Entamoeba histolytica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 1D-myo-inositol-1,3,4-trisphosphate
Entamoeba histolytica
-
ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
-
-
?
ATP + 1D-myo-inositol-1,3,4-trisphosphate
Entamoeba histolytica
-
ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Entamoeba histolytica
Q9XYQ1
-
-
Purification (Commentary)
Commentary
Organism
recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography and gel filtration
Entamoeba histolytica
Reaction
Reaction
Commentary
Organism
ATP + 1D-myo-inositol-1,3,4-trisphosphate = ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
determination of substrate binding sites, catalytic reaction mechanism
Entamoeba histolytica
ATP + 1D-myo-inositol-1,3,4-trisphosphate = ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
determination of substrate binding sites, catalytic reaction mechanism
Entamoeba histolytica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 1D-myo-inositol-1,3,4-trisphosphate
-
662834
Entamoeba histolytica
ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
-
-
-
?
ATP + 1D-myo-inositol-1,3,4-trisphosphate
-
662834
Entamoeba histolytica
ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
-
-
-
?
additional information
the enzyme shows multiple activities
662834
Entamoeba histolytica
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Entamoeba histolytica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Entamoeba histolytica
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Entamoeba histolytica
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of GST-tagged wild-type enzyme and of mutant enzymes in Escherichia coli
Entamoeba histolytica
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Entamoeba histolytica
Crystallization (Commentary) (protein specific)
Crystallization
Organism
purified recombinant enzyme free or in complex with ATP analogue AMP-PCP and substrate inositol-1,3,4-trisphosphate in presence of Mg2+, 30 mg/ml protein in 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, and 10 mM DTT, mixed with a solution containing 20% PEG 3000, 0.1 M Tris-HCl, pH 7.5, and 0.1 M Ca(OAc)2, several months, crystals are seeded into sitting drops of 15 mg/ml protein, 25% PEG 3350, 5% PEG 400, 0.1 M Bis-Tris, pH 5.5, 10 mM DTT, and 10 mM L-cysteine, with or without ligands added as 10 mM AMP-PCP, 10 mM MgCl2, and 5 mM inositol-1,3,4-trisphosphate, direct X-ray diffraction analysis after this step or further seeding adding ADP and other ligands, overview, X-ray diffraction structure determination and analysis at 1.2-2.0 A resolution
Entamoeba histolytica
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of diverse mutants and analysis of the function of the residues in catalysis and substrate binding, overview
Entamoeba histolytica
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Entamoeba histolytica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 1D-myo-inositol-1,3,4-trisphosphate
Entamoeba histolytica
-
ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
-
-
?
ATP + 1D-myo-inositol-1,3,4-trisphosphate
Entamoeba histolytica
-
ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography and gel filtration
Entamoeba histolytica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 1D-myo-inositol-1,3,4-trisphosphate
-
662834
Entamoeba histolytica
ADP + 1D-myo-inositol-1,3,4,5-tetrakisphosphate
-
-
-
?
ATP + 1D-myo-inositol-1,3,4-trisphosphate
-
662834
Entamoeba histolytica
ADP + 1D-myo-inositol-1,3,4,6-tetrakisphosphate
-
-
-
?
additional information
the enzyme shows multiple activities
662834
Entamoeba histolytica
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Entamoeba histolytica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Entamoeba histolytica
Other publictions for EC 2.7.1.159
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738303
Chen
Natural genetic variation of s ...
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Front. Plant Sci.
7
1070
2016
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2
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737332
Tang
Arabidopsis inositol 1,3,4-tri ...
Arabidopsis thaliana
Acta Biochim. Biophys. Sin. (Shanghai)
45
549-560
2013
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1
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1
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2
2
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723655
Zhang
Regulation of inositol 1,3,4-t ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
109
2290-2295
2012
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1
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723438
Du
Characterization of an inosito ...
Oryza sativa
Plant Mol. Biol.
77
547-563
2011
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701020
Wilson
Neural tube defects in mice wi ...
Mus musculus
Proc. Natl. Acad. Sci. USA
106
9831-9835
2009
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685755
Niu
OsITL1 gene encoding an inosit ...
Oryza sativa
Biotechnol. Lett.
30
1687-1692
2008
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690054
Saiardi
Human ITPK1: a reversible inos ...
Homo sapiens
Sci. Signal.
1
1-3
2008
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9
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1
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9
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673698
Sweetman
Arabidopsis thaliana inositol ...
Arabidopsis thaliana
FEBS Lett.
581
4165-4171
2007
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1
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1
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14
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6
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8
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14
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2
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17
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4
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4
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674860
Chamberlain
Integration of inositol phosph ...
Homo sapiens
J. Biol. Chem.
282
28117-28125
2007
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659473
Verbsky
The pathway for the production ...
Homo sapiens
J. Biol. Chem.
280
1911-1920
2005
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662834
Miller
Specificity determinants in in ...
Entamoeba histolytica
Mol. Cell
18
201-212
2005
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663162
Qin
The Arabidopsis inositol 1,3,4 ...
Arabidopsis thaliana
Plant Physiol. Biochem.
43
947-954
2005
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1
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662164
Sun
Inositol 1,3,4-trisphosphate 5 ...
Homo sapiens
J. Biol. Chem.
278
43645-43653
2003
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1
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1
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1
1
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662121
Sun
Inositol 1,3,4-trisphosphate 5 ...
Homo sapiens
J. Biol. Chem.
277
45759-45764
2002
1
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1
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1
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662110
Wilson
Inositol 1,3,4-trisphosphate 5 ...
Bos taurus, Homo sapiens
J. Biol. Chem.
276
40998-41004
2001
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1
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3
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662796
Field
An Entamoeba histolytica inosi ...
Entamoeba histolytica, Entamoeba histolytica HM-1
Mol. Biochem. Parasitol.
108
119-123
2000
1
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660847
Wilson
Characterization of a cDNA enc ...
Arabidopsis thaliana
Biochem. Biophys. Res. Commun.
232
678-681
1997
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1
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1
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640816
Wilson
Isolation of inositol 1,3,4-tr ...
Bos taurus, Homo sapiens
J. Biol. Chem.
271
11904-11910
1996
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2
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2
2
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1
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662090
Abdullah
Purification and characterizat ...
Rattus norvegicus
J. Biol. Chem.
267
22340-22345
1992
1
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1
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1
-
-
-
-
1
-
3
1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-