BRENDA - Enzyme Database show
show all sequences of 2.7.1.157

Kidney N-acetylgalactosamine (GalNAc)-1-phosphate kinase, a new pathway of GalNAc activation

Pastuszak, I.; Drake, R.; Elbein, A.D.; J. Biol. Chem. 271, 20776-20782 (1996)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.063
-
ATP
-
Sus scrofa
0.14
-
N-acetyl-D-galactosamine
-
Sus scrofa
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Sus scrofa
5829
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
divalent cation required, efficiency in descending order: Mg2+ > Mn2+ > Co2+, optimum Co2+ concentration is 10 mM, maximal activity is 50% of that with Mg2+
Sus scrofa
Mg2+
divalent cation required, efficiency in descending order: Mg2+ > Mn2+ > Co2+, optimum Mg2+ concentration is 5 mM
Sus scrofa
Mn2+
divalent cation required, efficiency in descending order: Mg2+ > Mn2+ > Co2+, optimum Mn2+ concentration is 2-8 mM, maximal activity is 50% of that with Mg2+
Sus scrofa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
gel filtration
Sus scrofa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Oryctolagus cuniculus
-
-
-
Sus scrofa
-
-
-
Purification (Commentary)
Commentary
Organism
-
Sus scrofa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
-
Oryctolagus cuniculus
-
kidney
highest total and specific activity
Sus scrofa
-
kidney
-
Homo sapiens
-
liver
-
Oryctolagus cuniculus
-
liver
-
Sus scrofa
-
lung
-
Sus scrofa
-
additional information
no activity in pancreas, brain, aorta, heart
Sus scrofa
-
spleen
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.43
-
-
Sus scrofa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-phosphate + N-acetyl-alpha-D-galactosamine
8% of the specific activity with ATP
659129
Sus scrofa
acetate + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
-
659129
Homo sapiens
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
-
659129
Sus scrofa
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
-
659129
Oryctolagus cuniculus
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-D-galactosamine
-
659129
Sus scrofa
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
-
ITP + N-acetyl-alpha-D-galactosamine
9% of the specific activity with ATP
659129
Sus scrofa
IDP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
phosphoenolpyruvate + N-acetyl-alpha-D-galactosamine
11% of the specific activity with ATP
659129
Sus scrofa
pyruvate + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 50000, SDS-PAGE
Sus scrofa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
9
-
Sus scrofa
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
10
pH 6.0: about 45% of maximal activity, pH 10.0: about 35% of maximal activity
Sus scrofa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.063
-
ATP
-
Sus scrofa
0.14
-
N-acetyl-D-galactosamine
-
Sus scrofa
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Sus scrofa
5829
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
divalent cation required, efficiency in descending order: Mg2+ > Mn2+ > Co2+, optimum Co2+ concentration is 10 mM, maximal activity is 50% of that with Mg2+
Sus scrofa
Mg2+
divalent cation required, efficiency in descending order: Mg2+ > Mn2+ > Co2+, optimum Mg2+ concentration is 5 mM
Sus scrofa
Mn2+
divalent cation required, efficiency in descending order: Mg2+ > Mn2+ > Co2+, optimum Mn2+ concentration is 2-8 mM, maximal activity is 50% of that with Mg2+
Sus scrofa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
gel filtration
Sus scrofa
Purification (Commentary) (protein specific)
Commentary
Organism
-
Sus scrofa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
-
Oryctolagus cuniculus
-
kidney
highest total and specific activity
Sus scrofa
-
kidney
-
Homo sapiens
-
liver
-
Oryctolagus cuniculus
-
liver
-
Sus scrofa
-
lung
-
Sus scrofa
-
additional information
no activity in pancreas, brain, aorta, heart
Sus scrofa
-
spleen
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.43
-
-
Sus scrofa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-phosphate + N-acetyl-alpha-D-galactosamine
8% of the specific activity with ATP
659129
Sus scrofa
acetate + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
-
659129
Homo sapiens
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
-
659129
Sus scrofa
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-alpha-D-galactosamine
-
659129
Oryctolagus cuniculus
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-D-galactosamine
-
659129
Sus scrofa
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
-
ITP + N-acetyl-alpha-D-galactosamine
9% of the specific activity with ATP
659129
Sus scrofa
IDP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
phosphoenolpyruvate + N-acetyl-alpha-D-galactosamine
11% of the specific activity with ATP
659129
Sus scrofa
pyruvate + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 50000, SDS-PAGE
Sus scrofa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
9
-
Sus scrofa
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
10
pH 6.0: about 45% of maximal activity, pH 10.0: about 35% of maximal activity
Sus scrofa
Other publictions for EC 2.7.1.157
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721309
Kristiansson
N-acetylgalactosamine kinase: ...
Homo sapiens
Appl. Biochem. Biotechnol.
166
57-63
2012
-
-
1
-
7
-
-
36
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
21
-
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
36
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
704525
Stockbridge
The intrinsic reactivity of AT ...
Homo sapiens
J. Biol. Chem.
284
22747-22757
2009
-
-
-
-
-
-
-
3
-
1
-
1
-
2
-
-
-
1
-
-
-
-
1
-
1
-
-
2
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
2
1
1
-
-
-
-
-
-
-
-
709300
Agnew
Mechanistic studies on human N ...
Homo sapiens
J. Enzyme Inhib. Med. Chem.
25
370-376
2009
-
-
1
-
-
-
3
3
-
1
-
1
-
4
-
-
1
1
-
-
-
-
3
-
-
-
-
3
-
2
-
1
1
-
-
-
-
2
1
-
-
-
-
4
1
4
-
1
-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
659494
Thoden
The molecular architecture of ...
Homo sapiens
J. Biol. Chem.
280
32784-32791
2005
-
-
1
1
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672583
Bourgeaux
Two-step enzymatic synthesis o ...
Homo sapiens
Bioorg. Med. Chem. Lett.
15
5459-5462
2005
-
1
1
-
-
-
-
1
-
-
1
-
-
3
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659129
Pastuszak
Kidney N-acetylgalactosamine ( ...
Homo sapiens, Oryctolagus cuniculus, Sus scrofa
J. Biol. Chem.
271
20776-20782
1996
-
-
-
-
-
-
-
2
1
3
1
-
-
5
-
-
1
-
-
8
1
-
7
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
3
1
-
-
-
-
1
-
8
1
-
7
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
659130
Pastuszak
Identification of the GalNAc k ...
Homo sapiens, Sus scrofa
J. Biol. Chem.
271
23653-23656
1996
-
-
1
-
-
-
-
2
2
-
1
-
-
5
-
-
-
-
-
2
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
2
-
1
-
-
-
-
-
-
2
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-