Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 (DE3) cells | Leishmania donovani |
Crystallization (Comment) | Organism |
---|---|
crystals obtained by the hanging-drop vapour-diffusion method at 23°C diffract to 1.95 A resolution and belong to the hexagonal space group P61, with unit-cell parameters a = b = 100.25, c = 126.77 A | Leishmania donovani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.116 | - |
ATP | pH 8.5, 25°C | Leishmania donovani | |
0.296 | - |
D-ribose | pH 8.5, 25°C | Leishmania donovani |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
SDS-PAGE | Leishmania donovani |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose | Leishmania donovani | the enzyme catalyzes the first step of ribose metabolism | ADP + D-ribose 5-phosphate | - |
? | |
ATP + D-ribose | Leishmania donovani BPK282A1 | the enzyme catalyzes the first step of ribose metabolism | ADP + D-ribose 5-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | E9BIX7 | - |
- |
Leishmania donovani BPK282A1 | E9BIX7 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-affinity and gel-filtration chromatography | Leishmania donovani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose | - |
Leishmania donovani | ADP + D-ribose 5-phosphate | - |
? | |
ATP + D-ribose | the enzyme catalyzes the first step of ribose metabolism | Leishmania donovani | ADP + D-ribose 5-phosphate | - |
? | |
ATP + D-ribose | - |
Leishmania donovani BPK282A1 | ADP + D-ribose 5-phosphate | - |
? | |
ATP + D-ribose | the enzyme catalyzes the first step of ribose metabolism | Leishmania donovani BPK282A1 | ADP + D-ribose 5-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, SDS-PAGE | Leishmania donovani |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Leishmania donovani |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
44 | - |
Tm-value | Leishmania donovani |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.15 | - |
ATP | pH 8.5, 25°C | Leishmania donovani | |
2.28 | - |
D-ribose | pH 8.5, 25°C | Leishmania donovani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Leishmania donovani |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme catalyzes the first step of ribose metabolism | Leishmania donovani |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.7 | - |
D-ribose | pH 8.5, 25°C | Leishmania donovani | |
18.5 | - |
ATP | pH 8.5, 25°C | Leishmania donovani |