Cloned (Comment) | Organism |
---|---|
expressed in COS-1 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C347A | by site-directed mutagenesis, mutation severely impairs localization at the Golgi complex resulting in cytosolic accumulation instead | Homo sapiens |
C351A | by site-directed mutagenesis, mutation severely impairs localization at the Golgi complex resulting in cytosolic accumulation instead | Homo sapiens |
C354A | by site-directed mutagenesis | Homo sapiens |
DELTA1-123 | production by site-directed mutagenesis, localization in cytosol | Homo sapiens |
DELTA124-537 | production by site-directed mutagenesis, resulted in almost complete accumulation in the nucleus | Homo sapiens |
DELTA219-496 | production by site-directed mutagenesis, localization in cytosol and nucleus | Homo sapiens |
DELTA340-537 | production by site-directed mutagenesis, significant accumulation into the nucleus, existence of nuclear export signals in the C-terminal part of enzyme, traditional nuclear export signals 511-IEVRVHCQLVRL-522 in the CC3 domain and a class 2 nuclear export signals 347-CRAGCFVC-354 between the CC1 and the CC2 domains | Homo sapiens |
DELTA454-537 | production by site-directed mutagenesis, localization in cytosol and nucleus | Homo sapiens |
DELTA514-537 | production by site-directed mutagenesis, nuclear localization of ceramide kinase | Homo sapiens |
DELTA520-537 | production by site-directed mutagenesis, localization is mostly cytosolic but is also detected in the nucleus | Homo sapiens |
DELTA525-537 | production by site-directed mutagenesis, both localization and cellular activity are lost | Homo sapiens |
DELTA528-537 | production by site-directed mutagenesis, no effect of localization and activity when assaying at the cellular level with exogenously added substrate, activity is lost after cell lysis in vitro | Homo sapiens |
DELTA533-537 | production by site-directed mutagenesis, no effect of localization and activity | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Golgi complex | - |
Homo sapiens | - |
- |
additional information | truncation of the PH domain either from the N-terminus or C-terminus results in disruption of the PH domain fold and loss of wild type ceramide kinase localization | Homo sapiens | - |
- |
nucleus | - |
Homo sapiens | 5634 | - |
plasma membrane | - |
Homo sapiens | 5886 | - |
vesicle | microtubule-driven vesicles that traffic between the Golgi complex and the plasma membrane | Homo sapiens | 31982 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ceramide | Homo sapiens | - |
ADP + ceramide 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8TCT0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ceramide | - |
Homo sapiens | ADP + ceramide 1-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ceramide kinase | - |
Homo sapiens |
CERK | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Homo sapiens |