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Literature summary for 2.7.1.11 extracted from
- Sequence, expression, and characterization of the first archaeal ATP-dependent 6-phosphofructokinase, a non-allosteric enzyme related to the phosphofructokinase-B sugar kinase family, from the hyperthermophilic crenarchaeote Aeropyrum pernix (2001), Arch. Microbiol., 177, 62-69.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ammonium sulfate | 50 mM, 50% inhibition | Aeropyrum pernix |  |
| KCl | 100 mM, 50% inhibition | Aeropyrum pernix | |
| NaCl | 1 M, 50% inhibition | Aeropyrum pernix | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
ATP | pH 6.7, 85°C | Aeropyrum pernix | |
| 0.6 | - |
D-glucose 6-phosphate | pH 6.7, 85°C | Aeropyrum pernix | |
| 0.68 | - |
D-fructose 6-phosphate | pH 6.7, 85°C | Aeropyrum pernix | |
| 1 | - |
adenosine | pH 6.7, 85°C | Aeropyrum pernix | |
| 1 | - |
fructose | pH 6.7, 85°C | Aeropyrum pernix | |
| 3 | - |
D-ribose 5-phosphate | pH 6.7, 85°C | Aeropyrum pernix | |
| 5 | - |
D-ribose | pH 6.7, 85°C | Aeropyrum pernix | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Aeropyrum pernix | |
| Mg2+ | required for activity | Aeropyrum pernix | |
| Mn2+ | 9% of activity with Mg2+ | Aeropyrum pernix | |
| Ni2+ | 15% of activity with Mg2+ | Aeropyrum pernix | |
| Zn2+ | 46% of activity with Mg2+ | Aeropyrum pernix | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33392 | - |
alpha4, x * 33392, deduced from nucleotide sequence | Aeropyrum pernix |
| 115000 | - |
gel filtration | Aeropyrum pernix |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose 6-phosphate | Aeropyrum pernix | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aeropyrum pernix | Q9YG89 | hyperthermophilic crenarchaeote | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant PFK, Bio-Scale Q5, gel filtration | Aeropyrum pernix |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + adenosine | - |
Aeropyrum pernix | ADP + ? | - |
? | |
| ATP + D-fructose 6-phosphate | - |
Aeropyrum pernix | ADP + D-fructose 1,6-bisphosphate | - |
? | |
| ATP + fructose | - |
Aeropyrum pernix | ADP + fructose 1-phosphate | - |
? | |
| ATP + glucose 6-phosphate | - |
Aeropyrum pernix | ADP + glucose 1,6-bisphosphate | - |
? | |
| ATP + ribose | - |
Aeropyrum pernix | ADP + ribose 1-phosphate | - |
? | |
| ATP + ribose 5-phosphate | - |
Aeropyrum pernix | ADP + ribose 1,5-bisphosphate | - |
? | |
| GTP + D-fructose 6-phosphate | 76% of activity with ATP | Aeropyrum pernix | GDP + D-fructose 1,6-bisphosphate | - |
? | |
| ITP + D-fructose 6-phosphate | 76% of activity with ATP | Aeropyrum pernix | IDP + D-fructose 1,6-bisphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | alpha4, x * 33392, deduced from nucleotide sequence | Aeropyrum pernix |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Aeropyrum pernix |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 100 | very low activity at 40°C and 100°C, approx. 50% of maximal activity at 75°C, activation energy: 57 kJ/mol | Aeropyrum pernix |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
no loss of activity after 2 h | Aeropyrum pernix |
| 95 | - |
complete loss of activity after 2 h | Aeropyrum pernix |
| 100 | - |
complete loss of activity after 30 min, 10% and 45% loss of activity after 30 min in the presence of 1 M KCl and NaCl, respectively | Aeropyrum pernix |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | - |
- |
Aeropyrum pernix |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | 60% of maximal activity at pH 5.0 and pH 8.5 | Aeropyrum pernix |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
ammonium sulfate | pH 6.7, 85°C | Aeropyrum pernix | |
| 0.1 | - |
KCl | pH 6.7, 85°C | Aeropyrum pernix | |
| 1 | - |
NaCl | pH 6.7, 85°C | Aeropyrum pernix | |
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