Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | activation | Saccharomyces cerevisiae | |
ADP | slight | Saccharomyces cerevisiae | |
D-fructose 2,6-bisphosphate | strong positive allosteric action on PFK | Saccharomyces cerevisiae | |
NH4+ | increases maximum activity of PFK and the affinity of PFK to fructose 6-phosphate | Saccharomyces cerevisiae | |
phosphate | synergistic with AMP | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
dilution inactivates | Saccharomyces cerevisiae |
dilution leads to enzyme dissociation | Saccharomyces cerevisiae |
PMSF stabilizes during purification | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae | |
citrate | cAMP, ADP or fructose 1,6-bisphosphate restore activity | Saccharomyces cerevisiae | |
MgATP2- | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pH-dependence of kinetic properties of cytosolic and plastid isozymes | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Saccharomyces cerevisiae | |
Mg2+ | required for activity | Saccharomyces cerevisiae | |
Mg2+ | MgATP is the active substrate | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
112000 | - |
alpha2beta4alpha2 or beta2alpha4beta2, 4 * 118000 + 4 * 112000, SDS-PAGE | Saccharomyces cerevisiae |
118000 | - |
alpha2beta4alpha2 or beta2alpha4beta2, 4 * 118000 + 4 * 112000, SDS-PAGE | Saccharomyces cerevisiae |
835000 | - |
sedimentation equilibrium analysis | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | Saccharomyces cerevisiae | dominant rate-controlling enzyme of glucose degradation via Embden-Meyerhof pathway, involved in generation of glycolytic oscillations | ADP + D-fructose 1,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
baker's yeast, preferred source: pitching yeast | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Cibacron Blue F3G-A, gel filtration, ion-exchange | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + beta-D-fructofuranose 6-phosphate = ADP + beta-D-fructofuranose 1,6-bisphosphate | mechanism | Saccharomyces cerevisiae | |
ATP + beta-D-fructofuranose 6-phosphate = ADP + beta-D-fructofuranose 1,6-bisphosphate | structure-oriented allosteric four-state model describing substrate and effector actions on PFK | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
0°C, dilute enzyme solution in 5 mM imidazole-HCl buffer, pH 7, 1 mM D-fructose 6-phosphate, 5 mM 2-mercaptoethanol, 0.5 mM PMSF, 6 h, 20% loss of activity | Saccharomyces cerevisiae |
4°C, concentrated enzyme solution, 50 mM potassium phosphate buffer, pH 7, 1 mM EDTA, 5 mM 2-mercaptoethanol, 0.5 mM PMSF, 1 mM fructose 6-phosphate, several months | Saccharomyces cerevisiae |
room temperature, 20% loss of activity within 1 month | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Saccharomyces cerevisiae | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | dominant rate-controlling enzyme of glucose degradation via Embden-Meyerhof pathway, involved in generation of glycolytic oscillations | Saccharomyces cerevisiae | ADP + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | alpha2beta4alpha2 or beta2alpha4beta2, 4 * 118000 + 4 * 112000, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH-dependence of kinetic properties | Saccharomyces cerevisiae |
7 | - |
optimum depends on the ATP and fructose 6-phosphate concentration | Saccharomyces cerevisiae |