Cloned (Comment) | Organism |
---|---|
cDNA encoding HK I is cloned and expressed in M+R42 cells | Rattus norvegicus |
mutant HK I is cloned and expressed in M+R42 cells | Rattus norvegicus |
Crystallization (Comment) | Organism |
---|---|
hexokinase I from brain complexed with glucose and phosphate | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
D532E | HK I mutant | Rattus norvegicus |
D532K | HK I mutant | Rattus norvegicus |
D532N | HK I mutant | Rattus norvegicus |
D84A | HK I mutant with 2fold increased Ki value for the inhibitory 1,5-anhydro-D-glucitol 6-phosphate, mutation diminishes the ability of phosphate to antagonize inhibition, but has no effect on the inhibition by phosphate at higher concentrations | Rattus norvegicus |
D84E | HK I mutant, slightly increased Ki value for the inhibitory 1,5-anhydro-D-glucitol 6-phosphate, antagonistic effect of phosphate is drastically reduced, no effect on the inhibition by phosphate at higher concentrations | Rattus norvegicus |
D84K | HK I mutant, increased Ki value for the inhibitory 1,5-anhydro-D-glucitol 6-phosphate, antagonistic effect of phosphate is abolished, slightly decreased Ki value for inhibition by phosphate at higher concentrations | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,5-Anhydro-D-glucitol 6-phosphate | hexokinase I, antagonism by phosphate at low concentrations | Rattus norvegicus | |
D-glucose 6-phosphate | product inhibition of hexokinase I, antagonism by phosphate at low concentrations results from competition for a common anion binding site located in the N-terminal half | Rattus norvegicus | |
phosphate | competitive versus ATP; hexokinase I, at high concentrations | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | 0.038 | D-glucose | recombinant wild-type hexokinase I, D84A, D84E and D84K mutants | Rattus norvegicus | |
0.43 | 0.47 | ATP | recombinant wild-type hexokinase I, D84A, D84E and D84K mutants | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P05708 | recombinant hexokinase type I | - |
Purification (Comment) | Organism |
---|---|
hexokinase I from brain | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | hexokinase I | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ATP | - |
Rattus norvegicus | ADP + D-glucose 6-phosphate | - |
? | |
additional information | structure model of hexokinase I | Rattus norvegicus | ? | - |
? | |
additional information | allosteric regulation of hexokinase I, the functional regulatory glucose 6-phosphate binding site is located in the N-terminal half | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | in the crystal structure | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
1,5-Anhydro-D-glucitol 6-phosphate | recombinant wild-type hexokinase I | Rattus norvegicus | |
0.018 | - |
1,5-Anhydro-D-glucitol 6-phosphate | recombinant D84E mutant hexokinase I | Rattus norvegicus | |
0.028 | - |
1,5-Anhydro-D-glucitol 6-phosphate | recombinant D84K mutant hexokinase I | Rattus norvegicus | |
0.033 | - |
1,5-Anhydro-D-glucitol 6-phosphate | recombinant D84A mutant hexokinase I | Rattus norvegicus | |
13 | - |
phosphate | recombinant D84K mutant hexokinase I | Rattus norvegicus | |
15 | - |
phosphate | recombinant D84E mutant hexokinase I | Rattus norvegicus | |
17 | - |
phosphate | recombinant wild-type hexokinase I | Rattus norvegicus | |
18 | - |
phosphate | recombinant D84A mutant hexokinase I | Rattus norvegicus |