Cloned (Comment) | Organism |
---|---|
hexokinase cDNA is cloned, sequenced and expressed in Escherichia coli as a fusion protein including an N-terminal polyhistidine tag and enterokinase cleavage site | Schistosoma mansoni |
General Stability | Organism |
---|---|
monothioglycerol stabilizes, enzyme activity is highly dependent on maintenance of free SH-groups | Schistosoma mansoni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,5-Anhydro-D-glucitol 6-phosphate | less effective than glucose 6-phosphate, competitive versus ATP | Schistosoma mansoni | |
D-glucose 6-phosphate | competitive versus ATP; moderate inhibition | Schistosoma mansoni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
D-glucose | at pH 8.5 | Schistosoma mansoni | |
0.93 | - |
ATP | at pH 8.5 | Schistosoma mansoni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50400 | - |
x * 55000, calculated from the amino acid sequence, x * 50400, 52 kDa with His-tag at the N-terminus, SDS-PAGE | Schistosoma mansoni |
55000 | - |
x * 55000, calculated from the amino acid sequence, x * 50400, 52 kDa with His-tag at the N-terminus, SDS-PAGE | Schistosoma mansoni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schistosoma mansoni | - |
recombinant hexokinase | - |
Purification (Comment) | Organism |
---|---|
recombinant hexokinase, expressed in Escherichia coli | Schistosoma mansoni |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Schistosoma mansoni |
Storage Stability | Organism |
---|---|
-80°C, 20 mM Hepes, 1 mM EDTA, 20 mM glucose, 10 mM monothioglycerol, pH 7.5, several months, stable | Schistosoma mansoni |
4°C, several days, progressive loss of activity | Schistosoma mansoni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + ATP | - |
Schistosoma mansoni | ADP + D-glucose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, calculated from the amino acid sequence, x * 50400, 52 kDa with His-tag at the N-terminus, SDS-PAGE | Schistosoma mansoni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Schistosoma mansoni |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | variations within the range have no substantial effect on stability during purification procedure | Schistosoma mansoni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Schistosoma mansoni |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
D-glucose 6-phosphate | at pH 8.5 | Schistosoma mansoni | |
0.5 | - |
1,5-Anhydro-D-glucitol 6-phosphate | at pH 8.5 | Schistosoma mansoni |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Schistosoma mansoni | analytical isoelectric focusing | - |
6 |