BRENDA - Enzyme Database
show all sequences of 2.6.1.97

Discovery and characterization of an amidinotransferase involved in the modification of archaeal tRNA

Phillips, G.; Chikwana, V.; Maxwell, A.; El-Yacoubi, B.; Swairjo, M.; Iwata-Reuyl, D.; De Crecy-Lagard, V.; J. Biol. Chem. 285, 12706-12713 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene mj1022
Methanocaldococcus jannaschii
gene tgtA2
Haloferax volcanii
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of and genotype and phenotype of the DELTA tgtA2 strain VDC5203
Haloferax volcanii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
Haloferax volcanii
-
L-glutamate + archaeine15 in tRNA
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
Methanocaldococcus jannaschii
-
L-glutamate + archaeine15 in tRNA
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
Methanocaldococcus jannaschii DSM 2661
-
L-glutamate + archaeine15 in tRNA
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
Haloferax volcanii H26
-
L-glutamate + archaeine15 in tRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Haloferax volcanii
-
gene tgtA2
-
Haloferax volcanii H26
-
gene tgtA2
-
Methanocaldococcus jannaschii
Q58428
gene mj1022
-
Methanocaldococcus jannaschii DSM 2661
Q58428
gene mj1022
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
-
719859
Haloferax volcanii
L-glutamate + archaeine15 in tRNA
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
-
719859
Methanocaldococcus jannaschii
L-glutamate + archaeine15 in tRNA
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
preQ0-tRNASer is used as substrate
719859
Haloferax volcanii
L-glutamate + archaeine15 in tRNA
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
preQ0-tRNASer is used as substrate
719859
Methanocaldococcus jannaschii
L-glutamate + archaeine15 in tRNA
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
-
719859
Methanocaldococcus jannaschii DSM 2661
L-glutamate + archaeine15 in tRNA
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
preQ0-tRNASer is used as substrate
719859
Methanocaldococcus jannaschii DSM 2661
L-glutamate + archaeine15 in tRNA
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
-
719859
Haloferax volcanii H26
L-glutamate + archaeine15 in tRNA
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
preQ0-tRNASer is used as substrate
719859
Haloferax volcanii H26
L-glutamate + archaeine15 in tRNA
-
-
-
?
additional information
recombinant MjTgtA2 converts preQ0-tRNA to G+-tRNA using several nitrogen sources and to do so in an ATP-independent process. MjTgtA2 shows in vitro glutaminase activity
719859
Methanocaldococcus jannaschii
?
-
-
-
-
additional information
recombinant MjTgtA2 converts preQ0-tRNA to G+-tRNA using several nitrogen sources and to do so in an ATP-independent process. MjTgtA2 shows in vitro glutaminase activity
719859
Methanocaldococcus jannaschii DSM 2661
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
glutaminase activity assay at
Methanocaldococcus jannaschii
Cloned(Commentary) (protein specific)
Commentary
Organism
gene mj1022
Methanocaldococcus jannaschii
gene tgtA2
Haloferax volcanii
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of and genotype and phenotype of the DELTA tgtA2 strain VDC5203
Haloferax volcanii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
Haloferax volcanii
-
L-glutamate + archaeine15 in tRNA
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
Methanocaldococcus jannaschii
-
L-glutamate + archaeine15 in tRNA
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
Methanocaldococcus jannaschii DSM 2661
-
L-glutamate + archaeine15 in tRNA
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
Haloferax volcanii H26
-
L-glutamate + archaeine15 in tRNA
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
-
719859
Haloferax volcanii
L-glutamate + archaeine15 in tRNA
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
-
719859
Methanocaldococcus jannaschii
L-glutamate + archaeine15 in tRNA
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
preQ0-tRNASer is used as substrate
719859
Haloferax volcanii
L-glutamate + archaeine15 in tRNA
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
preQ0-tRNASer is used as substrate
719859
Methanocaldococcus jannaschii
L-glutamate + archaeine15 in tRNA
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
-
719859
Methanocaldococcus jannaschii DSM 2661
L-glutamate + archaeine15 in tRNA
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
preQ0-tRNASer is used as substrate
719859
Methanocaldococcus jannaschii DSM 2661
L-glutamate + archaeine15 in tRNA
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
-
719859
Haloferax volcanii H26
L-glutamate + archaeine15 in tRNA
-
-
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
preQ0-tRNASer is used as substrate
719859
Haloferax volcanii H26
L-glutamate + archaeine15 in tRNA
-
-
-
?
additional information
recombinant MjTgtA2 converts preQ0-tRNA to G+-tRNA using several nitrogen sources and to do so in an ATP-independent process. MjTgtA2 shows in vitro glutaminase activity
719859
Methanocaldococcus jannaschii
?
-
-
-
-
additional information
recombinant MjTgtA2 converts preQ0-tRNA to G+-tRNA using several nitrogen sources and to do so in an ATP-independent process. MjTgtA2 shows in vitro glutaminase activity
719859
Methanocaldococcus jannaschii DSM 2661
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
glutaminase activity assay at
Methanocaldococcus jannaschii
General Information
General Information
Commentary
Organism
evolution
structure-based alignments comparing arcTGT and TgtA2 reveal that TgtA2 lacks key arcTGT catalytic residues and contains an additional module. Members of the TgtA2 and arcTGT family do not perfectly co-distribute
Haloferax volcanii
evolution
structure-based alignments comparing arcTGT and TgtA2 reveal that TgtA2 lacks key arcTGT catalytic residues and contains an additional module. Members of the TgtA2 and arcTGT family do not perfectly co-distribute
Methanocaldococcus jannaschii
malfunction
a Haloferax volcanii DELTAtgtA2 derivative demonstrates that tRNA from the mutant strain lacks G+ and instead accumulates preQ0
Haloferax volcanii
malfunction
a Haloferax volcanii DELTAtgtA2 derivative demonstrates that tRNA from the mutant strain lacks G+ and instead accumulates preQ0
Methanocaldococcus jannaschii
metabolism
TgtA2 is involved in archaeosine biosynthesis in vivo. Archaeosine biosynthesis is especially complex, involving the initial production of 7-cyano-7-deazaguanine (preQ0), an advanced precursor that is produced in a tRNA-independent portion of the biosynthesis, followed by its insertion into the tRNA by the enzyme tRNA-guanine transglycosylase, which replaces the target guanine base yielding preQ0-tRNA
Haloferax volcanii
metabolism
TgtA2 is involved in archaeosine biosynthesis in vivo. Archaeosine biosynthesis is especially complex, involving the initial production of 7-cyano-7-deazaguanine (preQ0), an advanced precursor that is produced in a tRNA-independent portion of the biosynthesis, followed by its insertion into the tRNA by the enzyme tRNA-guanine transglycosylase, which replaces the target guanine base yielding preQ0-tRNA
Methanocaldococcus jannaschii
additional information
presence of the 7-deazaguanosine derivative archaeosine, i.e. G+, at position 15 in tRNA is one of the diagnostic molecular characteristics of the archaea
Haloferax volcanii
additional information
presence of the 7-deazaguanosine derivative archaeosine, i.e. G+, at position 15 in tRNA is one of the diagnostic molecular characteristics of the archaea
Methanocaldococcus jannaschii
General Information (protein specific)
General Information
Commentary
Organism
evolution
structure-based alignments comparing arcTGT and TgtA2 reveal that TgtA2 lacks key arcTGT catalytic residues and contains an additional module. Members of the TgtA2 and arcTGT family do not perfectly co-distribute
Haloferax volcanii
evolution
structure-based alignments comparing arcTGT and TgtA2 reveal that TgtA2 lacks key arcTGT catalytic residues and contains an additional module. Members of the TgtA2 and arcTGT family do not perfectly co-distribute
Methanocaldococcus jannaschii
malfunction
a Haloferax volcanii DELTAtgtA2 derivative demonstrates that tRNA from the mutant strain lacks G+ and instead accumulates preQ0
Haloferax volcanii
malfunction
a Haloferax volcanii DELTAtgtA2 derivative demonstrates that tRNA from the mutant strain lacks G+ and instead accumulates preQ0
Methanocaldococcus jannaschii
metabolism
TgtA2 is involved in archaeosine biosynthesis in vivo. Archaeosine biosynthesis is especially complex, involving the initial production of 7-cyano-7-deazaguanine (preQ0), an advanced precursor that is produced in a tRNA-independent portion of the biosynthesis, followed by its insertion into the tRNA by the enzyme tRNA-guanine transglycosylase, which replaces the target guanine base yielding preQ0-tRNA
Haloferax volcanii
metabolism
TgtA2 is involved in archaeosine biosynthesis in vivo. Archaeosine biosynthesis is especially complex, involving the initial production of 7-cyano-7-deazaguanine (preQ0), an advanced precursor that is produced in a tRNA-independent portion of the biosynthesis, followed by its insertion into the tRNA by the enzyme tRNA-guanine transglycosylase, which replaces the target guanine base yielding preQ0-tRNA
Methanocaldococcus jannaschii
additional information
presence of the 7-deazaguanosine derivative archaeosine, i.e. G+, at position 15 in tRNA is one of the diagnostic molecular characteristics of the archaea
Haloferax volcanii
additional information
presence of the 7-deazaguanosine derivative archaeosine, i.e. G+, at position 15 in tRNA is one of the diagnostic molecular characteristics of the archaea
Methanocaldococcus jannaschii
Other publictions for EC 2.6.1.97
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
717018
Phillips
Diversity of archaeosine synth ...
Euryarchaeota, no activity in Pyrobaculum calidifontis, no activity in Pyrobaculum islandicum, no activity in Sulfolobus acidocaldarius, Saccharolobus solfataricus, Saccharolobus solfataricus P2, Sulfurisphaera tokodaii
ACS Chem. Biol.
7
300-305
2012
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719859
Phillips
Discovery and characterization ...
Haloferax volcanii, Haloferax volcanii H26, Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
J. Biol. Chem.
285
12706-12713
2010
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